Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jviromet.2008.01.006
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dc.titleThe VP1 protein of avian encephalomyelitis virus is a major host-protective immunogen that serves as diagnostic potential
dc.contributor.authorWei, L.
dc.contributor.authorZhou, J.
dc.contributor.authorWang, J.
dc.contributor.authorShi, L.
dc.contributor.authorLiu, J.
dc.contributor.authorChee, L.L.
dc.contributor.authorWei, T.
dc.contributor.authorKwang, J.
dc.date.accessioned2011-07-26T07:05:25Z
dc.date.available2011-07-26T07:05:25Z
dc.date.issued2008
dc.identifier.citationWei, L., Zhou, J., Wang, J., Shi, L., Liu, J., Chee, L.L., Wei, T., Kwang, J. (2008). The VP1 protein of avian encephalomyelitis virus is a major host-protective immunogen that serves as diagnostic potential. Journal of Virological Methods 149 (1) : 56-62. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jviromet.2008.01.006
dc.identifier.issn01660934
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/24853
dc.description.abstractAvian encephalomyelitis virus (AEV) is an important pathogen of poultry and is classified as a member of Picornaviridae. To investigate the protective immunity induced by AEV structural proteins, recombinant VP1, VP0, and VP3 proteins were expressed in a baculovirus system. The result of in vivo protection assays shows that the VP1 protein is a major host-protective immunogen against AEV challenge and demonstrates further that the antibody raised against VP1 protein could neutralize more effectively AEV infection than antibody against VP3 or VP0 protein in a virus neutralization test. These purified recombinant proteins were subsequently evaluated as enzyme-linked immunosorbent assay (ELISA) antigens for detection of AEV infection. A total number of 50 positive sera and 30 negative sera were tested for ELISA validation. Results obtained by testing 193 sera from chickens suspected of being infected AEV further showed that the diagnostic sensitivities of the VP1, VP3, and VP0 protein-based ELISAs were 98.1, 80.6, and 51.9%, and their specificities were 100, 87.9, and 81.8%, respectively. Both sensitivity and specificity of the VP1 protein-based ELISA were comparable with a commercially available test, indicating that the VP1 protein has a highly promising and reliable diagnostic potential, and thus is a suitable antigen for ELISA detection of AEV antibodies in chickens. © 2008 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.jviromet.2008.01.006
dc.sourceScopus
dc.subjectAntigenicity
dc.subjectAvian encephalomyelitis virus (AEV)
dc.subjectDiagnostic potential
dc.subjectImmunogenicity
dc.subjectVP1 protein
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1016/j.jviromet.2008.01.006
dc.description.sourcetitleJournal of Virological Methods
dc.description.volume149
dc.description.issue1
dc.description.page56-62
dc.identifier.isiut000255300700008
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