Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41378-018-0003-8
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dc.titleMicrofluidics-enabled phenotyping of a whole population of C. elegans worms over their embryonic and post-embryonic development at single-organism resolution
dc.contributor.authorLetizia, Maria Cristina
dc.contributor.authorCornaglia, Matteo
dc.contributor.authorTrouillon, Raphael
dc.contributor.authorSorrentino, Vincenzo
dc.contributor.authorMouchiroud, Laurent
dc.contributor.authorSleiman, Maroun S Bou
dc.contributor.authorAuwerx, Johan
dc.contributor.authorGijs, Martin AM
dc.date.accessioned2024-04-09T04:40:19Z
dc.date.available2024-04-09T04:40:19Z
dc.date.issued2018-05-07
dc.identifier.citationLetizia, Maria Cristina, Cornaglia, Matteo, Trouillon, Raphael, Sorrentino, Vincenzo, Mouchiroud, Laurent, Sleiman, Maroun S Bou, Auwerx, Johan, Gijs, Martin AM (2018-05-07). Microfluidics-enabled phenotyping of a whole population of C. elegans worms over their embryonic and post-embryonic development at single-organism resolution. MICROSYSTEMS & NANOENGINEERING 4 (1). ScholarBank@NUS Repository. https://doi.org/10.1038/s41378-018-0003-8
dc.identifier.issn2055-7434
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/247798
dc.description.abstractThe organism Caenorhabditis elegans is a performant model system for studying human biological processes and diseases, but until now all phenome data are produced as population-averaged read-outs. Monitoring of individual responses to drug treatments would however be more informative. Here, a new strategy to track different phenotypic traits of individual C. elegans nematodes throughout their full life-cycle—i.e., embryonic and post-embryonic development, until adulthood onset, differently from life-span—is presented. In an automated fashion, single worms were synchronized, isolated, and cultured from egg to adulthood in a microfluidic device, where their identity was preserved during their whole development. Several phenotypes were monitored and quantified for each animal, resulting in high-content phenome data. Specifically, the method was validated by analyzing the response of C. elegans to doxycycline, an antibiotic fairly well-known to prolong the development and activate mitochondrial stress-response pathways in different species. Interestingly, the obtained extensive single-worm phenome not only confirmed the dramatic doxycycline effect on the worm developmental delay, but more importantly revealed subtle yet severe treatment-dependent phenotypes that are representative of minority subgroups and would have otherwise stayed hidden in an averaged dataset. Such heterogeneous response started during the embryonic development, which makes essential having a dedicated chip that allows including this early developmental stage in the drug assay. Our approach would therefore allow elucidating pharmaceutical or therapeutic responses that so far were still being overlooked.
dc.language.isoen
dc.publisherSPRINGERNATURE
dc.sourceElements
dc.subjectScience & Technology
dc.subjectTechnology
dc.subjectNanoscience & Nanotechnology
dc.subjectInstruments & Instrumentation
dc.subjectScience & Technology - Other Topics
dc.subjectUNFOLDED PROTEIN RESPONSE
dc.subjectMITOCHONDRIAL-FUNCTION
dc.subjectBEHAVIOR
dc.subjectSYSTEM
dc.subjectDEVICE
dc.subjectPLATFORM
dc.typeArticle
dc.date.updated2024-04-08T10:14:23Z
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.1038/s41378-018-0003-8
dc.description.sourcetitleMICROSYSTEMS & NANOENGINEERING
dc.description.volume4
dc.description.issue1
dc.published.statePublished
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