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https://doi.org/10.1038/s41586-019-1192-5
Title: | Total synthesis of <i>Escherichia coli</i> with a recoded genome | Authors: | Fredens, Julius Wang, Kaihang de la Torre, Daniel Funke, Louise FH Robertson, Wesley E Christova, Yonka Chia, Tiongsun Schmied, Wolfgang H Dunkelmann, Daniel L Beranek, Vaclav Uttamapinant, Chayasith Llamazares, Andres Gonzalez Elliott, Thomas S Chin, Jason W |
Keywords: | Science & Technology Multidisciplinary Sciences Science & Technology - Other Topics PRECISE MANIPULATION MESSENGER-RNA CODON CHOICE STEM-LOOP GENE PROTEIN SEQUENCE RECOMBINATION REASSIGNMENT CHROMOSOMES |
Issue Date: | 23-May-2019 | Publisher: | NATURE RESEARCH | Citation: | Fredens, Julius, Wang, Kaihang, de la Torre, Daniel, Funke, Louise FH, Robertson, Wesley E, Christova, Yonka, Chia, Tiongsun, Schmied, Wolfgang H, Dunkelmann, Daniel L, Beranek, Vaclav, Uttamapinant, Chayasith, Llamazares, Andres Gonzalez, Elliott, Thomas S, Chin, Jason W (2019-05-23). Total synthesis of Escherichia coli with a recoded genome. NATURE 569 (7757) : 514-518. ScholarBank@NUS Repository. https://doi.org/10.1038/s41586-019-1192-5 | Abstract: | Nature uses 64 codons to encode the synthesis of proteins from the genome, and chooses 1 sense codon—out of up to 6 synonyms—to encode each amino acid. Synonymous codon choice has diverse and important roles, and many synonymous substitutions are detrimental. Here we demonstrate that the number of codons used to encode the canonical amino acids can be reduced, through the genome-wide substitution of target codons by defined synonyms. We create a variant of Escherichia coli with a four-megabase synthetic genome through a high-fidelity convergent total synthesis. Our synthetic genome implements a defined recoding and refactoring scheme—with simple corrections at just seven positions—to replace every known occurrence of two sense codons and a stop codon in the genome. Thus, we recode 18,214 codons to create an organism with a 61-codon genome; this organism uses 59 codons to encode the 20 amino acids, and enables the deletion of a previously essential transfer RNA. | Source Title: | NATURE | URI: | https://scholarbank.nus.edu.sg/handle/10635/247587 | ISSN: | 0028-0836 1476-4687 |
DOI: | 10.1038/s41586-019-1192-5 |
Appears in Collections: | Staff Publications Elements |
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Total synthesis of Escherichia coli with a recoded genome.pdf | Accepted version | 3.9 MB | Adobe PDF | OPEN | Post-print | View/Download |
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