Please use this identifier to cite or link to this item:
https://doi.org/10.1007/7651_2018_163
DC Field | Value | |
---|---|---|
dc.title | Full-thickness human skin equivalent models of atopic dermatitis | |
dc.contributor.author | Sriram, G | |
dc.contributor.author | Bigliardi, PL | |
dc.contributor.author | Bigliardi-Qi, M | |
dc.date.accessioned | 2024-03-20T07:30:05Z | |
dc.date.available | 2024-03-20T07:30:05Z | |
dc.date.issued | 2019-01-01 | |
dc.identifier.citation | Sriram, G, Bigliardi, PL, Bigliardi-Qi, M (2019-01-01). Full-thickness human skin equivalent models of atopic dermatitis. Methods in Molecular Biology 1879 : 367-383. ScholarBank@NUS Repository. https://doi.org/10.1007/7651_2018_163 | |
dc.identifier.isbn | 9781493988693 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/247531 | |
dc.description.abstract | Atopic dermatitis is a chronic inflammatory skin disease caused by complex multifactorial etiology. In the recent years, there have been significant advances in tissue engineering and the generation of in vitro skin models representative of healthy and diseased states. This chapter describes the methodology for the fabrication of in vitro human skin equivalent (HSE) from human keratinocytes and fibroblasts using a fibrin-based dermal matrix and serum-free culture conditions. Modification of the culture conditions with the supplementation of Th2 cytokines such as interleukin-4 induces the development of atopic dermatitis-like skin model. The chapter also describes the histological and immunohistochemical tools for characterization of the HSE model. The reconstruction of tissue-engineered HSE models that recapitulate the essential features of atopic dermatitis provides powerful tools for deeper understanding of the underlying pathological mechanisms on epidermal level, identification and testing of novel treatment options, and safety and toxicological evaluation in a pathophysiologically relevant system. | |
dc.publisher | Springer New York | |
dc.source | Elements | |
dc.subject | 3D tissue constructs | |
dc.subject | Atopic dermatitis | |
dc.subject | Fibrin | |
dc.subject | Full-thickness | |
dc.subject | Human skin equivalent | |
dc.subject | Organoids | |
dc.subject | Organotypic culture | |
dc.subject | Tissue engineering | |
dc.subject | Cells, Cultured | |
dc.subject | Cytokines | |
dc.subject | Dermatitis, Atopic | |
dc.subject | Fibrin | |
dc.subject | Fibroblasts | |
dc.subject | Humans | |
dc.subject | Keratinocytes | |
dc.subject | Models, Biological | |
dc.subject | Skin | |
dc.subject | Skin, Artificial | |
dc.subject | Tissue Engineering | |
dc.type | Book Chapter | |
dc.date.updated | 2024-03-20T06:54:13Z | |
dc.contributor.department | DEAN'S OFFICE (DENTISTRY) | |
dc.contributor.department | MEDICINE | |
dc.description.doi | 10.1007/7651_2018_163 | |
dc.description.sourcetitle | Methods in Molecular Biology | |
dc.description.volume | 1879 | |
dc.description.page | 367-383 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
Show simple item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
Full-thickness human skin equivalent models of atopic dermatitis.pdf | Accepted version | 684.33 kB | Adobe PDF | OPEN | Post-print | View/Download |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.