Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.antiviral.2007.01.004
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dc.titleEnhanced potency and efficacy of 29-mer shRNAs in inhibition of Enterovirus 71
dc.contributor.authorTan, E.L.
dc.contributor.authorChow, V.T.K.
dc.contributor.authorPoh, C.L.
dc.contributor.authorTan, T.M.C.
dc.date.accessioned2011-07-26T06:52:48Z
dc.date.available2011-07-26T06:52:48Z
dc.date.issued2007
dc.identifier.citationTan, E.L., Chow, V.T.K., Poh, C.L., Tan, T.M.C. (2007). Enhanced potency and efficacy of 29-mer shRNAs in inhibition of Enterovirus 71. Antiviral Research 74 (1) : 9-15. ScholarBank@NUS Repository. https://doi.org/10.1016/j.antiviral.2007.01.004
dc.identifier.issn01663542
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/24667
dc.description.abstractEnterovirus 71 (EV71) is the main causative agent of hand, foot, and mouth disease (HFMD) in young children. It has been associated with severe neurological complications and has caused significant mortalities in large-scale outbreaks in Asia. In this study, we demonstrated an enhanced silencing of EV71 through the use of chemically synthesized 29-mer shRNAs. The 29-mer shRNAs were designed to target three highly conserved regions of EV71 genome. Transfection of rhabdomyosarcoma (RD) cells with the 29-mer shRNAs significantly inhibited EV71 replication in a dose-dependent manner as demonstrated by reduction of viral RNA, VP1 protein and plaque forming units. The inhibitory effects were more potent and were achieved at 10-fold lower concentrations when compared to 19-mer siRNAs reported previously [Sim, A.C.N., Luhur, A., Tan, T.M.C., Chow, V.T.K., Poh, C.L., 2005. RNA interference against Enterovirus 71 infection. Virology 341, 72-79]. The viral inhibitory effects lasted 72 h post-infection and there was no adverse off-target silencing effect. Gene silencing by 29-mer shRNAs targeted at the 3Dpol region (sh-3D) was the most effective, achieving 91% viral inhibition. Further evaluation found that no enhanced inhibitory effects were observed when sh-3D was cotransfected with each of the other two candidates. This study showed an improvement in triggering RNAi using the more potent 29-mer shRNAs, indicating its therapeutic potential against EV71. © 2007 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.antiviral.2007.01.004
dc.sourceScopus
dc.subject29-mer shRNAs
dc.subjectEnhanced silencing effects
dc.subjectEnterovirus 71
dc.subjectHand, foot and mouth disease (HFMD)
dc.subjectPotential antiviral therapy
dc.subjectRNA interference
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1016/j.antiviral.2007.01.004
dc.description.sourcetitleAntiviral Research
dc.description.volume74
dc.description.issue1
dc.description.page9-15
dc.identifier.isiut000245614400002
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