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|Title:||LuxS/autoinducer-2 quorum sensing molecule regulates transcriptional virulence gene expression in Clostridium difficile||Authors:||Lee, A.S.Y.
|Issue Date:||2005||Citation:||Lee, A.S.Y., Song, K.P. (2005). LuxS/autoinducer-2 quorum sensing molecule regulates transcriptional virulence gene expression in Clostridium difficile. Biochemical and Biophysical Research Communications 335 (3) : 659-666. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2005.07.131||Abstract:||Toxigenic Clostridium difficile CCUG19126 produces the autoinducer-2 (AI-2) quorum sensing molecule that induces bioluminescence in Vibrio harveyi BB170 reporter strain. AI-2-containing cell-free supernatants from mid-log phase C. difficile and Escherichia coli DH5α expressing recombinant luxS cd upregulated the transcript levels of tcdA (7-10-fold), tcdB (4-6-fold), and tcdE (2-3-fold) in early-log C. difficile. In contrast, no induction occurred when cells were exposed to sterile medium or to cell-free supernatant from E. coli DH5α. AI-2 did not significantly increase the level of toxin A in early-log C. difficile. These results suggest that LuxS cd-dependent signaling regulates virulence gene expression at the transcriptional level in C. difficile. © 2005 Elsevier Inc. All rights reserved.||Source Title:||Biochemical and Biophysical Research Communications||URI:||http://scholarbank.nus.edu.sg/handle/10635/24653||ISSN:||0006291X||DOI:||10.1016/j.bbrc.2005.07.131|
|Appears in Collections:||Staff Publications|
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