Please use this identifier to cite or link to this item:
|Title:||LuxS/autoinducer-2 quorum sensing molecule regulates transcriptional virulence gene expression in Clostridium difficile||Authors:||Lee, A.S.Y.
|Issue Date:||2005||Citation:||Lee, A.S.Y., Song, K.P. (2005). LuxS/autoinducer-2 quorum sensing molecule regulates transcriptional virulence gene expression in Clostridium difficile. Biochemical and Biophysical Research Communications 335 (3) : 659-666. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2005.07.131||Abstract:||Toxigenic Clostridium difficile CCUG19126 produces the autoinducer-2 (AI-2) quorum sensing molecule that induces bioluminescence in Vibrio harveyi BB170 reporter strain. AI-2-containing cell-free supernatants from mid-log phase C. difficile and Escherichia coli DH5α expressing recombinant luxS cd upregulated the transcript levels of tcdA (7-10-fold), tcdB (4-6-fold), and tcdE (2-3-fold) in early-log C. difficile. In contrast, no induction occurred when cells were exposed to sterile medium or to cell-free supernatant from E. coli DH5α. AI-2 did not significantly increase the level of toxin A in early-log C. difficile. These results suggest that LuxS cd-dependent signaling regulates virulence gene expression at the transcriptional level in C. difficile. © 2005 Elsevier Inc. All rights reserved.||Source Title:||Biochemical and Biophysical Research Communications||URI:||http://scholarbank.nus.edu.sg/handle/10635/24653||ISSN:||0006291X||DOI:||10.1016/j.bbrc.2005.07.131|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Sep 26, 2020
WEB OF SCIENCETM
checked on Sep 18, 2020
checked on Sep 22, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.