Please use this identifier to cite or link to this item: https://doi.org/10.3389/fphys.2019.00272
Title: Optogenetic Monitoring of the Glutathione Redox State in Engineered Human Myocardium
Authors: Trautsch, Irina
Heta, Eriona
Soong, Poh Loong 
Levent, Elif
Nikolaev, Viacheslav O
Bogeski, Ivan
Katschinski, Doerthe M
Mayr, Manuel
Zimmermann, Wolfram-Hubertus
Keywords: Science & Technology
Life Sciences & Biomedicine
Physiology
optogenetics
engineered human myocardium
redox-reporters
stem cells
cardiomyocytes
fibroblasts
roGFP
GSH
HYDROGEN-PEROXIDE
HEART
PHYSIOLOGY
CALCIUM
H2O2
Issue Date: 4-Apr-2019
Publisher: FRONTIERS MEDIA SA
Citation: Trautsch, Irina, Heta, Eriona, Soong, Poh Loong, Levent, Elif, Nikolaev, Viacheslav O, Bogeski, Ivan, Katschinski, Doerthe M, Mayr, Manuel, Zimmermann, Wolfram-Hubertus (2019-04-04). Optogenetic Monitoring of the Glutathione Redox State in Engineered Human Myocardium. FRONTIERS IN PHYSIOLOGY 10. ScholarBank@NUS Repository. https://doi.org/10.3389/fphys.2019.00272
Abstract: Redox signaling affects all aspects of cardiac function and homeostasis. With the development of genetically encoded fluorescent redox sensors, novel tools for the optogenetic investigation of redox signaling have emerged. Here, we sought to develop a human heart muscle model for in-tissue imaging of redox alterations. For this, we made use of (1) the genetically-encoded Grx1-roGFP2 sensor, which reports changes in cellular glutathione redox status (GSH/GSSG), (2) human embryonic stem cells (HES2), and (3) the engineered heart muscle (EHM) technology. We first generated HES2 lines expressing Grx1-roGFP2 in cytosol or mitochondria compartments by TALEN-guided genomic integration. Grx1-roGFP2 sensor localization and function was verified by fluorescence imaging. Grx1-roGFP2 HES2 were then subjected to directed differentiation to obtain high purity cardiomyocyte populations. Despite being able to report glutathione redox potential from cytosol and mitochondria, we observed dysfunctional sarcomerogenesis in Grx1-roGFP2 expressing cardiomyocytes. Conversely, lentiviral transduction of Grx1-roGFP2 in already differentiated HES2-cardiomyocytes and human foreskin fibroblast was possible, without compromising cell function as determined in EHM from defined Grx1-roGFP2-expressing cardiomyocyte and fibroblast populations. Finally, cell-type specific GSH/GSSG imaging was demonstrated in EHM. Collectively, our observations suggests a crucial role for redox signaling in cardiomyocyte differentiation and provide a solution as to how this apparent limitation can be overcome to enable cell-type specific GSH/GSSG imaging in a human heart muscle context.
Source Title: FRONTIERS IN PHYSIOLOGY
URI: https://scholarbank.nus.edu.sg/handle/10635/245773
ISSN: 1664-042X
DOI: 10.3389/fphys.2019.00272
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