Please use this identifier to cite or link to this item: https://doi.org/10.1007/978-1-0716-3222-2_23
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dc.titleLaboratory Scale Production of Complex Proteins Using Charge Complimentary Nanoenvironments
dc.contributor.authorVallerinteavide Mavelli, G
dc.contributor.authorSadeghi, S
dc.contributor.authorDrum, CL
dc.date.accessioned2023-10-31T02:50:32Z
dc.date.available2023-10-31T02:50:32Z
dc.date.issued2023-01-01
dc.identifier.citationVallerinteavide Mavelli, G, Sadeghi, S, Drum, CL (2023-01-01). Laboratory Scale Production of Complex Proteins Using Charge Complimentary Nanoenvironments. Methods Mol Biol 2671 : 403-418. ScholarBank@NUS Repository. https://doi.org/10.1007/978-1-0716-3222-2_23
dc.identifier.issn1064-3745
dc.identifier.issn1940-6029
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/245638
dc.description.abstractProtein refolding is a crucial procedure in bacterial recombinant expression. Aggregation and misfolding are the two challenges that can affect the overall yield and specific activity of the folded proteins. We demonstrated the in vitro use of nanoscale “thermostable exoshells” (tES) to encapsulate, fold and release diverse protein substrates. With tES, the soluble yield, functional yield, and specific activity increased from 2-fold to >100-fold when compared to folding in its absence. On average, the soluble yield was determined to be 6.5 mg/100 mg of tES for a set of 12 diverse substrates evaluated. The electrostatic charge complementation between the tES interior and the protein substrate was considered as the primary determinant for functional folding. We thus describe a useful and simple method for in vitro folding that has been evaluated and implemented in our laboratory.
dc.publisherSpringer US
dc.sourceElements
dc.subjectIn vitro folding
dc.subjectInclusion bodies
dc.subjectProtein expression
dc.subjectProtein nanoparticles
dc.subjectProtein refolding
dc.subjectThermostable exoshells
dc.subjecttES
dc.subjectLaboratories
dc.subjectProtein Refolding
dc.subjectStatic Electricity
dc.typeArticle
dc.date.updated2023-10-30T03:06:15Z
dc.contributor.departmentMEDICINE
dc.description.doi10.1007/978-1-0716-3222-2_23
dc.description.sourcetitleMethods Mol Biol
dc.description.volume2671
dc.description.page403-418
dc.published.statePublished
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