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Title: A comparative analysis of scaffold material modifications for load-bearing applications in bone tissue engineering
Authors: Chim, H.
Lim, T.C.
Schantz, J.-T. 
Hutmacher, D.W. 
Chou, A.M.
Oliveira, A.L.
Reis, R.L.
Keywords: bone tissue engineering
fused deposition modelling
polymer scaffolds
Issue Date: 2006
Citation: Chim, H., Lim, T.C., Schantz, J.-T., Hutmacher, D.W., Chou, A.M., Oliveira, A.L., Reis, R.L. (2006). A comparative analysis of scaffold material modifications for load-bearing applications in bone tissue engineering. International Journal of Oral and Maxillofacial Surgery 35 (10) : 928-934. ScholarBank@NUS Repository.
Abstract: To facilitate optimal application of appropriate scaffold architectures for clinical trials, there is a need to compare different scaffold modifications under similar experimental conditions. In this study was assessed the effectiveness of poly-e-caprolactone (PCL) scaffolds fabricated by fused deposition modelling (FDM), with varying material modifications, for the purposes of bone tissue engineering. The incorporation of hydroxyapatite (HA) in PCL scaffolds, as well as precalcification through immersion in a simulated body fluid (SBF) to produce a biomimetic apatite coating on the scaffolds, was assessed. A series of in vitro studies spanning 3 weeks as well as in vivo studies utilizing a subcutaneous nude mouse model were carried out. PCL and HA-PCL scaffolds demonstrated increasing tissue growth extending throughout the implants, as well as superior mechanical strength and mineralization, as evidenced by X-ray imaging after 14 weeks in vivo. No significant difference was found between PCL and HA-PCL scaffolds. Precalcification with SBF did not result in increased osteoconductivity and cell proliferation as previously reported. Conversely, tensile forces exerted by tissue sheets bridging adjacent struts of the PCL scaffold caused flaking of the apatite coating that resulted in impaired cell attachment, growth and mineralization. The results suggest that scaffolds fabricated by FDM may have load-bearing applications. © 2006 International Association of Oral and Maxillofacial Surgeons.
Source Title: International Journal of Oral and Maxillofacial Surgery
ISSN: 09015027
DOI: 10.1016/j.ijom.2006.03.024
Appears in Collections:Staff Publications

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