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Title: Myoblast Transplantation for Cardiac Repair: From Automyoblast to Allomyoblast Transplantation
Authors: Guo, C.
Wang, C.
Haider, H.Kh.
Tan, R.-S. 
Shim, W.S.N. 
Wong, P.
Sim, E.K.W. 
Issue Date: 2008
Citation: Guo, C., Wang, C., Haider, H.Kh., Tan, R.-S., Shim, W.S.N., Wong, P., Sim, E.K.W. (2008). Myoblast Transplantation for Cardiac Repair: From Automyoblast to Allomyoblast Transplantation. Annals of Thoracic Surgery 86 (6) : 1841-1848. ScholarBank@NUS Repository.
Abstract: Background: We sought to compare host immune cell kinetics, survival profile of donor skeletal myoblasts, and skeletal myoblast graft efficacy after autologous and allogeneic skeletal myoblast transplantation into a rat model of myocardial infarction. Methods: One week after myocardial infarction, 128 animals were divided into four groups: group 1 (n = 24, receiving medium only), group 2 (n = 24, receiving medium and cyclosporine), group 3 (n = 40, autologous skeletal myoblast transplantation), and group 4 (n = 40, allogeneic skeletal myoblast transplantation with cyclosporine treatment). Rats were euthanized 10 minutes, 1 day, and 4, 7, and 28 days later. Host immune cell kinetics were assessed by immunohistochemical studies for macrophages, and CD4+ and CD8+ lymphocytes. Donor skeletal myoblast survival was confirmed by tracking prelabeled signals, and quantified by β-gal assay. Heart function was evaluated by echocardiography. Results: A transient immune cell infiltration was demonstrated in group 3, with macrophage infiltration on day 1 and day 4, CD8+ cell infiltration on day 4 and day 7, and CD4+ cell infiltration on day 4. In group 4, immunocyte infiltration was slightly more severe than that in group 3. Automyoblasts and allomyoblasts showed no significant difference of survival from day 1 to day 7 (p > 0.10); however, on day 28, automyoblasts showed better survival than allomyoblasts (p < 0.05). Transplantation of allomyoblasts increased systolic heart function and limited heart dilation after myocardial injury to a similar degree as automyoblasts (p > 0.10). Conclusions: The use of allomyoblasts is feasible and effective for cardiac repair with immunosuppressive treatment as compared with automyoblasts. © 2008 The Society of Thoracic Surgeons.
Source Title: Annals of Thoracic Surgery
ISSN: 00034975
DOI: 10.1016/j.athoracsur.2008.08.038
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