Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.scr.2008.02.002
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dc.titleReduction of myocardial infarct size by human mesenchymal stem cell conditioned medium
dc.contributor.authorTimmers, L.
dc.contributor.authorArslan, F.
dc.contributor.authorHoefer, I.E.
dc.contributor.authorDoevendans, P.A.
dc.contributor.authorPasterkamp, G.
dc.contributor.authorde, Kleijn D.P.V.
dc.contributor.authorLim, S.K.
dc.contributor.authorArmstrong, J.S.
dc.contributor.authorPiek, J.J.
dc.contributor.authorEl, Oakley R.M.
dc.contributor.authorChoo, A.
dc.contributor.authorLee, C.N.
dc.date.accessioned2011-07-19T10:13:28Z
dc.date.available2011-07-19T10:13:28Z
dc.date.issued2008
dc.identifier.citationTimmers, L., Arslan, F., Hoefer, I.E., Doevendans, P.A., Pasterkamp, G., de, Kleijn D.P.V., Lim, S.K., Armstrong, J.S., Piek, J.J., El, Oakley R.M., Choo, A., Lee, C.N. (2008). Reduction of myocardial infarct size by human mesenchymal stem cell conditioned medium. Stem Cell Research 1 (2) : 129-137. ScholarBank@NUS Repository. https://doi.org/10.1016/j.scr.2008.02.002
dc.identifier.issn18735061
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/24190
dc.description.abstractAlthough paracrine effects of mesenchymal stem cells (MSCs) have been suggested previously, cardioprotection by human MSC secretions has never been demonstrated. Human MSC-conditioned medium (CM) was collected by following a clinically compliant protocol. In a porcine model of ischemia and reperfusion injury, intravenous and intracoronary MSC-CM treatment significantly reduced myocardial nuclear oxidative stress as determined by immunostaining for 8-hydroxy-2′-deoxyguanosine. In addition, expression levels of phospho-SMAD2 and active caspase 3 were diminished following CM treatment, suggesting that TGF-β signaling and apoptosis were reduced. This was associated with a 60% reduction in infarct size and marked improvement of systolic and diastolic cardiac performance as assessed with echocardiography and pressure volume loops. Fractionation studies revealed that only the fraction of the CM containing products > 1000 kDa (100-220 nm) provided cardioprotection in a mouse model of ischemia and reperfusion injury. This indicates that the responsible paracrine factor of human MSCs is likely a large complex rather than a single small molecule. These data identify human MSC-CM as a promising therapeutic option to reduce myocardial infarct size in patients with acute MI and suggest that the use of stem cell secretions could extend the applicability of stem cells for therapeutic purposes. © 2008 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.scr.2008.02.002
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentSURGERY
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.1016/j.scr.2008.02.002
dc.description.sourcetitleStem Cell Research
dc.description.volume1
dc.description.issue2
dc.description.page129-137
dc.identifier.isiut000207571000006
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