Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bpj.2020.04.004
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dc.titleTo Hop or not to Hop: Exceptions in the FCS Diffusion Law
dc.contributor.authorGupta, Anjali
dc.contributor.authorPhang, Inn Yee
dc.contributor.authorWohland, Thorsten
dc.date.accessioned2023-06-07T04:36:59Z
dc.date.available2023-06-07T04:36:59Z
dc.date.issued2020-05-19
dc.identifier.citationGupta, Anjali, Phang, Inn Yee, Wohland, Thorsten (2020-05-19). To Hop or not to Hop: Exceptions in the FCS Diffusion Law. BIOPHYSICAL JOURNAL 118 (10) : 2434-2447. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bpj.2020.04.004
dc.identifier.issn0006-3495
dc.identifier.issn1542-0086
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/241635
dc.description.abstractDiffusion obstacles in membranes have not been directly visualized because of fast membrane dynamics and the occurrence of subresolution molecular complexes. To understand the obstacle characteristics, mobility-based methods are often used as an indirect way of assessing the membrane structure. Molecular movement in biological plasma membranes is often characterized by anomalous diffusion, but the exact underlying mechanisms are still elusive. Imaging total internal reflection fluorescence correlation spectroscopy (ITIR-FCS) is a well-established mobility-based method that provides spatially resolved diffusion coefficient maps and is combined with FCS diffusion law analysis to examine subresolution membrane organization. In recent years, although FCS diffusion law analysis has been instrumental in providing new insights into the membrane structure below the optical diffraction limit, there are certain exceptions and anomalies that require further clarification. To this end, we correlate the membrane structural features imaged by atomic force microscopy (AFM) with the dynamics measured using ITIR-FCS. We perform ITIR-FCS measurements on supported lipid bilayers (SLBs) of various lipid compositions to characterize the anomalous diffusion of lipid molecules in distinct obstacle configurations, along with the high-resolution imaging of the membrane structures with AFM. Furthermore, we validate our experimental results by performing simulations on image grids with experimentally determined obstacle configurations. This study demonstrates that FCS diffusion law analysis is a powerful tool to determine membrane heterogeneities implied from dynamics measurements. Our results corroborate the commonly accepted interpretations of imaging FCS diffusion law analysis, and we show that exceptions happen when domains reach the percolation threshold in a biphasic membrane and a network of domains behaves rather like a meshwork, resulting in hop diffusion.
dc.language.isoen
dc.publisherCELL PRESS
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectBiophysics
dc.subjectFLUORESCENCE CORRELATION SPECTROSCOPY
dc.subjectSUPPORTED LIPID-BILAYERS
dc.subjectATOMIC-FORCE MICROSCOPY
dc.subjectPLASMA-MEMBRANE
dc.subjectLATERAL DIFFUSION
dc.subjectPHASE-TRANSITION
dc.subjectLINE TENSION
dc.subjectDOMAINS
dc.subjectORGANIZATION
dc.subjectSEPARATION
dc.typeArticle
dc.date.updated2023-06-06T01:29:09Z
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1016/j.bpj.2020.04.004
dc.description.sourcetitleBIOPHYSICAL JOURNAL
dc.description.volume118
dc.description.issue10
dc.description.page2434-2447
dc.published.statePublished
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