Please use this identifier to cite or link to this item:
https://doi.org/10.4049/jimmunol.1502676
DC Field | Value | |
---|---|---|
dc.title | Blomia tropicalis-Specific TCR Transgenic Th2 Cells Induce Inducible BALT and Severe Asthma in Mice by an IL-4/IL-13-Dependent Mechanism | |
dc.contributor.author | Chua, Yen Leong | |
dc.contributor.author | Liong, Ka Hang | |
dc.contributor.author | Huang, Chiung-Hui | |
dc.contributor.author | Wong, Hok Sum | |
dc.contributor.author | Zhou, Qian | |
dc.contributor.author | Ler, Say Siong | |
dc.contributor.author | Tang, Yafang | |
dc.contributor.author | Low, Chin Pei | |
dc.contributor.author | Koh, Hui Yu | |
dc.contributor.author | Kuo, I-Chun | |
dc.contributor.author | Zhang, Yongliang | |
dc.contributor.author | Wong, WS Fred | |
dc.contributor.author | Peh, Hong Yong | |
dc.contributor.author | Lim, Hwee Ying | |
dc.contributor.author | Ge, Moyar Qing | |
dc.contributor.author | Haczku, Angela | |
dc.contributor.author | Angeli, Veronique | |
dc.contributor.author | MacAry, Paul A | |
dc.contributor.author | Chua, Kaw Yan | |
dc.contributor.author | Kemeny, David M | |
dc.date.accessioned | 2023-05-19T02:59:07Z | |
dc.date.available | 2023-05-19T02:59:07Z | |
dc.date.issued | 2016-11-15 | |
dc.identifier.citation | Chua, Yen Leong, Liong, Ka Hang, Huang, Chiung-Hui, Wong, Hok Sum, Zhou, Qian, Ler, Say Siong, Tang, Yafang, Low, Chin Pei, Koh, Hui Yu, Kuo, I-Chun, Zhang, Yongliang, Wong, WS Fred, Peh, Hong Yong, Lim, Hwee Ying, Ge, Moyar Qing, Haczku, Angela, Angeli, Veronique, MacAry, Paul A, Chua, Kaw Yan, Kemeny, David M (2016-11-15). Blomia tropicalis-Specific TCR Transgenic Th2 Cells Induce Inducible BALT and Severe Asthma in Mice by an IL-4/IL-13-Dependent Mechanism. JOURNAL OF IMMUNOLOGY 197 (10) : 3771-3781. ScholarBank@NUS Repository. https://doi.org/10.4049/jimmunol.1502676 | |
dc.identifier.issn | 0022-1767 | |
dc.identifier.issn | 1550-6606 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/239543 | |
dc.description.abstract | Previous studies have highlighted the importance of lung-draining lymph nodes in the respiratory allergic immune response, whereas the lung parenchymal immune system has been largely neglected. We describe a new in vivo model of respiratory sensitization to Blomia tropicalis, the principal asthma allergen in the tropics, in which the immune response is focused on the lung parenchyma by transfer of Th2 cells from a novel TCR transgenic mouse, specific for the major B. tropicalis allergen Blo t 5, that targets the lung rather than the draining lymph nodes. Transfer of highly polarized transgenic CD4 effector Th2 cells, termed BT-II, followed by repeated inhalation of Blo t 5 expands these cells in the lung >100-fold, and subsequent Blo t 5 challenge induced decreased body temperature, reduction in movement, and a fall in specific lung compliance unseen in conventional mouse asthma models following a physiological allergen challenge. These mice exhibit lung eosinophilia; smooth muscle cell, collagen, and goblet cell hyperplasia; hyper IgE syndrome; mucus plugging; and extensive inducible BALT. In addition, there is a fall in total lung volume and forced expiratory volume at 100 ms. These pathophysiological changes were substantially reduced and, in some cases, completely abolished by administration of neutralizing mAbs specific for IL-4 and IL-13 on weeks 1, 2, and 3. This IL-4/IL-13-dependent inducible BALT model will be useful for investigating the pathophysiological mechanisms that underlie asthma and the development of more effective drugs for treating severe asthma. | |
dc.language.iso | en | |
dc.publisher | AMER ASSOC IMMUNOLOGISTS | |
dc.source | Elements | |
dc.subject | Science & Technology | |
dc.subject | Life Sciences & Biomedicine | |
dc.subject | Immunology | |
dc.subject | ALLERGIC AIRWAY INFLAMMATION | |
dc.subject | BRONCHIAL LYMPHOID-TISSUE | |
dc.subject | SEVERE ATOPIC-DERMATITIS | |
dc.subject | REGULATORY T-CELLS | |
dc.subject | BROWN-NORWAY RATS | |
dc.subject | CROSS-REACTIVITY | |
dc.subject | MOUSE MODEL | |
dc.subject | MITE | |
dc.subject | SENSITIZATION | |
dc.subject | EXPOSURE | |
dc.type | Article | |
dc.date.updated | 2023-05-18T04:06:30Z | |
dc.contributor.department | MICROBIOLOGY AND IMMUNOLOGY | |
dc.contributor.department | PAEDIATRICS | |
dc.contributor.department | SAW SWEE HOCK SCHOOL OF PUBLIC HEALTH | |
dc.contributor.department | PHARMACOLOGY | |
dc.description.doi | 10.4049/jimmunol.1502676 | |
dc.description.sourcetitle | JOURNAL OF IMMUNOLOGY | |
dc.description.volume | 197 | |
dc.description.issue | 10 | |
dc.description.page | 3771-3781 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
Show simple item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
nihms-1782649.pdf | 2.24 MB | Adobe PDF | OPEN | None | View/Download |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.