Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.neulet.2007.02.082
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dc.titleProtein aggregate-containing neuron-like cells are differentiated from bone marrow mesenchymal stem cells from mice with neurofilament light subunit gene deficiency
dc.contributor.authorChao, Y.X.
dc.contributor.authorHe, B.P.
dc.contributor.authorCao, Q.
dc.contributor.authorTay, S.S.W.
dc.date.accessioned2011-07-18T08:44:47Z
dc.date.available2011-07-18T08:44:47Z
dc.date.issued2007
dc.identifier.citationChao, Y.X., He, B.P., Cao, Q., Tay, S.S.W. (2007). Protein aggregate-containing neuron-like cells are differentiated from bone marrow mesenchymal stem cells from mice with neurofilament light subunit gene deficiency. Neuroscience Letters 417 (3) : 240-245. ScholarBank@NUS Repository. https://doi.org/10.1016/j.neulet.2007.02.082
dc.identifier.issn03043940
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/23930
dc.description.abstractAutologous bone marrow mesenchymal stem cell (MSC) transplantation has great potential in cell therapy used for the treatment of neurodegenerative disorders. Since many genetic deficiencies have been reported in pathogenesis of the diseases, genetic backgrounds of donor stem cells should be concerned. In this study, effects of neurofilament light subunit (NFL) gene deficiency on proliferation and neuronal differentiation of MSCs were studied in vitro. Lower proliferation rate was observed in NFL-/- MSCs. When exposed to retinoic acid (RA), both NFL-/- and normal MSCs could express several markers of neuronal lineage, such as Nestin, MAP-2, NeuN, O4 and GFAP. However, the NFL expression at mRNA and protein levels was observed only in normal MSCs but absent in NFL-/- MSCs. Significant reductions in amount of neurofilament heavy subunit (NFH) protein and number of neuron-like cells were detected in differentiated NFL-/- MSCs. Interestingly, NFH positive protein accumulations were observed in the neuron-like cells derived from NFL-/- MSCs. These accumulations were perinuclear and morphologically similar to protein aggregations in motoneurons of the spinal cord in NFL-/- mice. The results suggest that NFL gene deficiency could retard MSCs proliferation and neuronal generation, even though the capability of neuronal lineage differentiation of MSCs may not be deterred. Moreover, the NFL-/- MSCs differentiated neuron-like cells carried on the genetic and pathologic deficiency, suggesting that the genetic quality of donor cells must not only be tested, but also modified before transplantation. This also points towards the possibility of creating a stem cell-derived cell model for pathogenesis study. © 2007 Elsevier Ireland Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.neulet.2007.02.082
dc.sourceScopus
dc.subjectMesenchymal stem cells
dc.subjectNeurofilament light subunit
dc.subjectNeuronal differentiation
dc.subjectProliferation
dc.typeArticle
dc.contributor.departmentANATOMY
dc.description.doi10.1016/j.neulet.2007.02.082
dc.description.sourcetitleNeuroscience Letters
dc.description.volume417
dc.description.issue3
dc.description.page240-245
dc.identifier.isiut000246666500005
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