Please use this identifier to cite or link to this item:
https://doi.org/10.1073/pnas.1913334117
DC Field | Value | |
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dc.title | Lck bound to coreceptor is less active than free Lck | |
dc.contributor.author | Wei, Qianru | |
dc.contributor.author | Brzostek, Joanna | |
dc.contributor.author | Sankaran, Shvetha | |
dc.contributor.author | Casas, Javier | |
dc.contributor.author | Hew, Lois Shi-Qi | |
dc.contributor.author | Yap, Jiawei | |
dc.contributor.author | Zhao, Xiang | |
dc.contributor.author | Wojciech, Lukasz | |
dc.contributor.author | Gascoigne, Nicholas RJ | |
dc.date.accessioned | 2023-05-11T02:27:32Z | |
dc.date.available | 2023-05-11T02:27:32Z | |
dc.date.issued | 2020-07-07 | |
dc.identifier.citation | Wei, Qianru, Brzostek, Joanna, Sankaran, Shvetha, Casas, Javier, Hew, Lois Shi-Qi, Yap, Jiawei, Zhao, Xiang, Wojciech, Lukasz, Gascoigne, Nicholas RJ (2020-07-07). Lck bound to coreceptor is less active than free Lck. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 117 (27) : 15809-15817. ScholarBank@NUS Repository. https://doi.org/10.1073/pnas.1913334117 | |
dc.identifier.issn | 0027-8424 | |
dc.identifier.issn | 1091-6490 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/239267 | |
dc.description.abstract | Src family kinase Lck plays critical roles during T cell development and activation, as it phosphorylates the TCR/CD3 complex to initiate TCR signaling. Lck is present either in coreceptor-bound or coreceptorunbound (free) forms, and we here present evidence that the two pools of Lck have different molecular properties. We discovered that the free Lck fraction exhibited higher mobility than CD8α-bound Lck in OT-I T hybridoma cells. The free Lck pool showed more activating Y394 phosphorylation than the coreceptor-bound Lck pool. Consistent with this, free Lck also had higher kinase activity, and free Lck mediated higher T cell activation as compared to coreceptor-bound Lck. Furthermore, the coreceptor-Lck coupling was independent of TCR activation. These findings give insights into the initiation of TCR signaling, suggesting that changes in coreceptor-Lck coupling constitute a mechanism for regulation of T cell sensitivity. | |
dc.language.iso | en | |
dc.publisher | NATL ACAD SCIENCES | |
dc.source | Elements | |
dc.subject | Science & Technology | |
dc.subject | Multidisciplinary Sciences | |
dc.subject | Science & Technology - Other Topics | |
dc.subject | Lck | |
dc.subject | coreceptor | |
dc.subject | T cell signaling | |
dc.subject | T cell receptor | |
dc.subject | T cell development | |
dc.subject | SRC-FAMILY KINASES | |
dc.subject | T-CELL DEVELOPMENT | |
dc.subject | TYROSINE KINASE | |
dc.subject | CD8 | |
dc.subject | SELECTION | |
dc.subject | ACTIVATION | |
dc.subject | GENERATION | |
dc.subject | THYMOCYTES | |
dc.subject | MECHANISM | |
dc.subject | DYNAMICS | |
dc.type | Article | |
dc.date.updated | 2023-05-10T08:29:11Z | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.contributor.department | MICROBIOLOGY AND IMMUNOLOGY | |
dc.description.doi | 10.1073/pnas.1913334117 | |
dc.description.sourcetitle | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | |
dc.description.volume | 117 | |
dc.description.issue | 27 | |
dc.description.page | 15809-15817 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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Lck bound to coreceptor is less active than free Lck.pdf | 1.56 MB | Adobe PDF | OPEN | Published | View/Download |
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