Please use this identifier to cite or link to this item: https://doi.org/10.3389/fphys.2022.1098408
DC FieldValue
dc.titleMultiple aspects of lymphatic dysfunction in an ApoE(-/-) mouse model of hypercholesterolemia
dc.contributor.authorDavis, Michael J
dc.contributor.authorScallan, Joshua P
dc.contributor.authorCastorena-Gonzalez, Jorge A
dc.contributor.authorKim, Hae Jin
dc.contributor.authorYing, Lim Hwee
dc.contributor.authorPin, Yeo Kim
dc.contributor.authorAngeli, Veronique
dc.date.accessioned2023-05-09T04:43:42Z
dc.date.available2023-05-09T04:43:42Z
dc.date.issued2023-01-06
dc.identifier.citationDavis, Michael J, Scallan, Joshua P, Castorena-Gonzalez, Jorge A, Kim, Hae Jin, Ying, Lim Hwee, Pin, Yeo Kim, Angeli, Veronique (2023-01-06). Multiple aspects of lymphatic dysfunction in an ApoE(-/-) mouse model of hypercholesterolemia. FRONTIERS IN PHYSIOLOGY 13. ScholarBank@NUS Repository. https://doi.org/10.3389/fphys.2022.1098408
dc.identifier.issn1664-042X
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/239248
dc.description.abstractIntroduction: Rodent models of cardiovascular disease have uncovered various types of lymphatic vessel dysfunction that occur in association with atherosclerosis, type II diabetes and obesity. Previously, we presented in vivo evidence for impaired lymphatic drainage in apolipoprotein E null (ApoE−/−) mice fed a high fat diet (HFD). Whether this impairment relates to the dysfunction of collecting lymphatics remains an open question. The ApoE−/− mouse is a well-established model of cardiovascular disease, in which a diet rich in fat and cholesterol on an ApoE deficient background accelerates the development of hypercholesteremia, atherosclerotic plaques and inflammation of the skin and other tissues. Here, we investigated various aspects of lymphatic function using ex vivo tests of collecting lymphatic vessels from ApoE+/+ or ApoE−/− mice fed a HFD. Methods: Popliteal collectors were excised from either strain and studied under defined conditions in which we could quantify changes in lymphatic contractile strength, lymph pump output, secondary valve function, and collecting vessel permeability. Results: Our results show that all these aspects of lymphatic vessel function are altered in deleterious ways in this model of hypercholesterolemia. Discussion: These findings extend previous in vivo observations suggesting significant dysfunction of lymphatic endothelial cells and smooth muscle cells from collecting vessels in association with a HFD on an ApoE-deficient background. An implication of our study is that collecting vessel dysfunction in this context may negatively impact the removal of cholesterol by the lymphatic system from the skin and the arterial wall and thereby exacerbate the progression and/or severity of atherosclerosis and associated inflammation.
dc.language.isoen
dc.publisherFRONTIERS MEDIA SA
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectPhysiology
dc.subjectlymphatic valve
dc.subjectpermeability
dc.subjectcontractile dysfunction
dc.subjecthigh fat diet
dc.subjectback-leak
dc.subjectpump limit
dc.subjectlymphatic muscle
dc.subjectlymphatic endothelium
dc.subjectDIET-INDUCED OBESITY
dc.subjectCHOLESTEROL
dc.subjectVESSELS
dc.subjectMICE
dc.subjectINCREASES
dc.subjectCONTRACTILITY
dc.subjectCONSTRICTION
dc.subjectVASCULATURE
dc.subjectMECHANISMS
dc.subjectINFECTION
dc.typeArticle
dc.date.updated2023-05-08T14:40:58Z
dc.contributor.departmentMICROBIOLOGY AND IMMUNOLOGY
dc.description.doi10.3389/fphys.2022.1098408
dc.description.sourcetitleFRONTIERS IN PHYSIOLOGY
dc.description.volume13
dc.published.statePublished
Appears in Collections:Staff Publications
Elements

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Multiple aspects of lymphatic dysfunction in an iApoEi supi--isup mouse model of hypercholesterolemia.pdf2.19 MBAdobe PDF

OPEN

PublishedView/Download

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.