Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/238906
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dc.titleDifferential immune responses of 3D gingival and periodontal connective tissue equivalents to microbial colonization
dc.contributor.authorMakkar, Hardik
dc.contributor.authorAtkuru, Srividya
dc.contributor.authorTang, Yi Ling
dc.contributor.authorSethi, Tanya
dc.contributor.authorLim, Chwee Teck
dc.contributor.authorTan, Kai Soo
dc.contributor.authorSriram, Gopu
dc.date.accessioned2023-04-19T03:03:11Z
dc.date.available2023-04-19T03:03:11Z
dc.date.issued2022-07-01
dc.identifier.citationMakkar, Hardik, Atkuru, Srividya, Tang, Yi Ling, Sethi, Tanya, Lim, Chwee Teck, Tan, Kai Soo, Sriram, Gopu (2022-07-01). Differential immune responses of 3D gingival and periodontal connective tissue equivalents to microbial colonization. JOURNAL OF TISSUE ENGINEERING 13. ScholarBank@NUS Repository.
dc.identifier.issn2041-7314
dc.identifier.issn2041-7314
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/238906
dc.description.abstractGingival and periodontal ligament fibroblasts are functionally distinct cell types within the dento-gingival unit that participate in host immune response. Their microenvironment influences the behavior and immune response to microbial challenge. We developed three-dimensional gingival and periodontal connective tissue equivalents (CTEs) using human fibrin-based matrix. The CTEs were characterized, and the heterogeneity in their innate immune response was investigated. The CTEs demonstrated no to minimal response to planktonic Streptococcus mitis and Streptococcus oralis, while their biofilms elicited a moderate increase in IL-6 and IL-8 production. In contrast, Fusobacterium nucleatum provoked a substantial increase in IL-6 and IL-8 production. Interestingly, the gingival CTEs secreted significantly higher IL-6, while periodontal counterparts produced higher IL-8. In conclusion, the gingival and periodontal CTEs exhibited differential responses to various bacterial challenges. This gives insights into the contribution of tissue topography and fibroblast heterogeneity in rendering protective and specific immune responses toward early biofilm colonizers.
dc.language.isoen
dc.publisherSAGE PUBLICATIONS INC
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectCell & Tissue Engineering
dc.subjectCell Biology
dc.subject3D culture
dc.subjecttissue constructs
dc.subjectfibroblast heterogeneity
dc.subjectinnate immune response
dc.subjectperiodontopathogens
dc.subjectinflammatory cytokines
dc.subjectFUSOBACTERIUM-NUCLEATUM BIOFILM
dc.subjectNF-KAPPA-B
dc.subjectLIGAMENT FIBROBLASTS
dc.subjectINFLAMMATORY RESPONSE
dc.subjectEPITHELIAL-CELLS
dc.subjectEXPRESSION
dc.subjectLIPOPOLYSACCHARIDE
dc.subjectCOMMENSAL
dc.subjectIL-6
dc.subjectHETEROGENEITY
dc.typeArticle
dc.date.updated2023-04-19T02:59:14Z
dc.contributor.departmentDEAN'S OFFICE (DENTISTRY)
dc.contributor.departmentBIOMEDICAL ENGINEERING
dc.description.sourcetitleJOURNAL OF TISSUE ENGINEERING
dc.description.volume13
dc.published.statePublished
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