Please use this identifier to cite or link to this item: https://doi.org/10.1002/adhm.202202376
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dc.titleModeling Crevicular Fluid Flow and Host-Oral Microbiome Interactions in a Gingival Crevice-on-Chip
dc.contributor.authorMakkar, Hardik
dc.contributor.authorZhou, Ying
dc.contributor.authorTan, Kai Soo
dc.contributor.authorLim, Chwee Teck
dc.contributor.authorSriram, Gopu
dc.date.accessioned2023-04-19T03:01:00Z
dc.date.available2023-04-19T03:01:00Z
dc.date.issued2022-11-28
dc.identifier.citationMakkar, Hardik, Zhou, Ying, Tan, Kai Soo, Lim, Chwee Teck, Sriram, Gopu (2022-11-28). Modeling Crevicular Fluid Flow and Host-Oral Microbiome Interactions in a Gingival Crevice-on-Chip. ADVANCED HEALTHCARE MATERIALS 12 (6). ScholarBank@NUS Repository. https://doi.org/10.1002/adhm.202202376
dc.identifier.issn2192-2640
dc.identifier.issn2192-2659
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/238905
dc.description.abstractGingival crevice and gingival crevicular fluid (GCF) flow play a crucial role at the gingiva-oral microbiome interface which contributes toward maintaining the balance between gingival health and periodontal disease. Interstitial flow of GCF strongly impacts the host-microbiome interactions and tissue responses. However, currently available in vitro preclinical models largely disregard the dynamic nature of gingival crevicular microenvironment, thus limiting the progress in the development of periodontal therapeutics. Here, a proof-of-principle “gingival crevice-on-chip” microfluidic platform to culture gingival connective tissue equivalent (CTE) under dynamic interstitial fluid flow mimicking the GCF is described. On-chip co-culture using oral symbiont (Streptococcus oralis) shows the potential to recapitulate microbial colonization, formation of biofilm-like structures at the tissue-microbiome interface, long-term co-culture, and bacterial clearance secondary to simulated GCF (s-GCF) flow. Further, on-chip exposure of the gingival CTEs to the toll-like receptor-2 (TLR-2) agonist or periodontal pathogen Fusobacterium nucleatum demonstrates the potential to mimic early gingival inflammation. In contrast to direct exposure, the induction of s-GCF flow toward the bacterial front attenuates the secretion of inflammatory mediators demonstrating the protective effect of GCF flow. This proposed in vitro platform offers the potential to study complex host-microbe interactions in periodontal disease and the development of periodontal therapeutics under near-microphysiological conditions.
dc.language.isoen
dc.publisherWILEY
dc.sourceElements
dc.subjectScience & Technology
dc.subjectTechnology
dc.subjectEngineering, Biomedical
dc.subjectNanoscience & Nanotechnology
dc.subjectMaterials Science, Biomaterials
dc.subjectEngineering
dc.subjectScience & Technology - Other Topics
dc.subjectMaterials Science
dc.subjectgingival crevices
dc.subjectgingival crevicular fluids
dc.subjecthost-microbe interactions
dc.subjectmicrofluidics
dc.subjectorgans-on-a-chip
dc.subjectperiodontal disease
dc.subjectFUSOBACTERIUM-NUCLEATUM BIOFILM
dc.subjectINTERSTITIAL FLOW
dc.subjectA-CHIP
dc.subjectSTAPHYLOCOCCUS-AUREUS
dc.subjectMORPHOGEN GRADIENTS
dc.subjectGLOBAL BURDEN
dc.subjectPERIODONTITIS
dc.subjectINFLAMMATION
dc.subjectPATHOGENESIS
dc.subjectDIFFERENTIATION
dc.typeArticle
dc.date.updated2023-04-19T02:56:29Z
dc.contributor.departmentDEAN'S OFFICE (DENTISTRY)
dc.contributor.departmentBIOMEDICAL ENGINEERING
dc.description.doi10.1002/adhm.202202376
dc.description.sourcetitleADVANCED HEALTHCARE MATERIALS
dc.description.volume12
dc.description.issue6
dc.published.statePublished
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