Please use this identifier to cite or link to this item: https://doi.org/10.3389/fped.2022.949756
DC FieldValue
dc.titleImmune and pathophysiologic profiling of antenatal coronavirus disease 2019 in the GIFT cohort: A Singaporean case-control study
dc.contributor.authorGu, Yue
dc.contributor.authorLow, Jia Ming
dc.contributor.authorTan, Jolene Su Yi
dc.contributor.authorNg, Melissa Shu Feng
dc.contributor.authorNg, Lisa FP
dc.contributor.authorShunmuganathan, Bhuvaneshwari
dc.contributor.authorGupta, Rashi
dc.contributor.authorMacAry, Paul AA
dc.contributor.authorAmin, Zubair
dc.contributor.authorLee, Le Ye
dc.contributor.authorLian, Derrick
dc.contributor.authorShek, Lynette Pei-Chi
dc.contributor.authorZhong, Youjia
dc.contributor.authorWang, Liang Wei
dc.date.accessioned2023-02-08T08:26:36Z
dc.date.available2023-02-08T08:26:36Z
dc.date.issued2022-09-15
dc.identifier.citationGu, Yue, Low, Jia Ming, Tan, Jolene Su Yi, Ng, Melissa Shu Feng, Ng, Lisa FP, Shunmuganathan, Bhuvaneshwari, Gupta, Rashi, MacAry, Paul AA, Amin, Zubair, Lee, Le Ye, Lian, Derrick, Shek, Lynette Pei-Chi, Zhong, Youjia, Wang, Liang Wei (2022-09-15). Immune and pathophysiologic profiling of antenatal coronavirus disease 2019 in the GIFT cohort: A Singaporean case-control study. FRONTIERS IN PEDIATRICS 10. ScholarBank@NUS Repository. https://doi.org/10.3389/fped.2022.949756
dc.identifier.issn2296-2360
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/236982
dc.description.abstractCOVID-19 can be severe in pregnant women, and have adverse consequences for the subsequent infant. We profiled the post-infectious immune responses in maternal and child blood as well as breast milk in terms of antibody and cytokine expression and performed histopathological studies on placentae obtained from mothers convalescent from antenatal COVID-19. Seventeen mother-child dyads (8 cases of antenatal COVID-19 and 9 healthy unrelated controls; 34 individuals in total) were recruited to the Gestational Immunity For Transfer (GIFT) study. Maternal and infant blood, and breast milk samples were collected over the first year of life. All samples were analyzed for IgG and IgA against whole SARS-CoV-2 spike protein, the spike receptor-binding domain (RBD), and previously reported immunodominant epitopes, as well as cytokine levels. The placentae were examined microscopically. The study is registered at clinicaltrials.gov under the identifier NCT04802278. We found high levels of virus-specific IgG in convalescent mothers and similarly elevated titers in newborn children. Thus, antenatal SARS-CoV-2 infection led to high plasma titers of virus-specific antibodies in infants postnatally. However, this waned within 3–6 months of life. Virus neutralization by plasma was not uniformly achieved, and the presence of antibodies targeting known immunodominant epitopes did not assure neutralization. Virus-specific IgA levels were variable among convalescent individuals’ sera and breast milk. Antibody transfer ratios and the decay of transplacentally transferred virus-specific antibodies in neonatal circulation resembled that for other pathogens. Convalescent mothers showed signs of chronic inflammation marked by persistently elevated IL17RA levels in their blood. Four placentae presented signs of acute inflammation, particularly in the subchorionic region, marked by neutrophil infiltration even though > 50 days had elapsed between virus clearance and delivery. Administration of a single dose of BNT162b2 mRNA vaccine to mothers convalescent from antenatal COVID-19 increased virus-specific IgG and IgA titers in breast milk, highlighting the importance of receiving the vaccine even after natural infection with the added benefit of enhanced passive immunity.
dc.language.isoen
dc.publisherFRONTIERS MEDIA SA
dc.sourceElements
dc.subjectSARS-CoV-2
dc.subjectantenatal COVID-19
dc.subjecttransplacental antibody transfer
dc.subjectbreast milk antibodies
dc.subjectplacental inflammation
dc.subjectGIFT
dc.typeArticle
dc.date.updated2023-02-08T06:25:20Z
dc.contributor.departmentMICROBIOLOGY AND IMMUNOLOGY
dc.contributor.departmentPAEDIATRICS
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.description.doi10.3389/fped.2022.949756
dc.description.sourcetitleFRONTIERS IN PEDIATRICS
dc.description.volume10
dc.published.statePublished
Appears in Collections:Staff Publications
Elements

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Immune and pathophysiologic profiling of antenatal coronavirus disease 2019 in the GIFT cohort A Singaporean case-control st.pdf2.64 MBAdobe PDF

OPEN

PublishedView/Download

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.