EFFICACY OF CORD LINING MESENCHYMAL STEM CELLS IN CUTANEOUS WOUND HEALING

Abstract

The purpose of this thesis is to investigate the effect of Cord Lining Mesenchymal Stem Cells (CL-MSCs) in accelerating wound healing. We utilised twenty-five db/db male mice and created two excisional wounds (10 mm x 10 mm) onto the dorsal aspect of each mouse (n=50). In addition, we used six hyperglycaemic-induced pigs, and created six full thickness dermal wounds (5 cm x 5 cm) onto the dorsal aspect of each pig (n=36). All animals were randomly divided into three arms; with CL-MSCs delivered through either (i) topical route or (ii) intraperitoneal route, and (iii) sham media administered for the control group. It is shown in our study that CL-MSCs accelerated wound healing in both db/db mice and hyperglycaemic pigs. Further, they enhanced tissue structure and formed collagen fibres that are larger in diameter. Their ability to modulate inflammatory cells and regulate the immune responses might be key contributors to this process.