Please use this identifier to cite or link to this item: https://doi.org/10.1038/s42003-021-01793-z
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dc.titleAssessment of transient changes in oxygen diffusion of single red blood cells using a microfluidic analytical platform
dc.contributor.authorChng, Kevin Ziyang
dc.contributor.authorNg, Yan Cheng
dc.contributor.authorNamgung, Bumseok
dc.contributor.authorTan, Justin Kok Soon
dc.contributor.authorPark, Soyeon
dc.contributor.authorTien, Sim Leng
dc.contributor.authorLeo, Hwa Liang
dc.contributor.authorKim, Sangho
dc.date.accessioned2022-10-26T09:04:41Z
dc.date.available2022-10-26T09:04:41Z
dc.date.issued2021-03-02
dc.identifier.citationChng, Kevin Ziyang, Ng, Yan Cheng, Namgung, Bumseok, Tan, Justin Kok Soon, Park, Soyeon, Tien, Sim Leng, Leo, Hwa Liang, Kim, Sangho (2021-03-02). Assessment of transient changes in oxygen diffusion of single red blood cells using a microfluidic analytical platform. Communications Biology 4 (1) : 271. ScholarBank@NUS Repository. https://doi.org/10.1038/s42003-021-01793-z
dc.identifier.issn2399-3642
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/233595
dc.description.abstractRed blood cells (RBCs) capability to deliver oxygen (O2) has been routinely measured by P50. Although this defines the ability of RBCs to carry O2 under equilibrium states, it cannot determine the efficacy of O2 delivery in dynamic blood flow. Here, we developed a microfluidic analytical platform (MAP) that isolates single RBCs for assessing transient changes in their O2 release rate. We found that in vivo (biological) and in vitro (blood storage) aging of RBC could lead to an increase in the O2 release rate, despite a decrease in P50. Rejuvenation of stored RBCs (Day 42), though increased the P50, failed to restore the O2 release rate to basal level (Day 0). The temporal dimension provided at the single-cell level by MAP could shed new insights into the dynamics of O2 delivery in both physiological and pathological conditions. © 2021, The Author(s).
dc.publisherNature Research
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceScopus OA2021
dc.typeArticle
dc.contributor.departmentOFFICE OF THE DEPUTY PRESIDENT(RES&TECH)
dc.contributor.departmentCOLLEGE OF DESIGN AND ENGINEERING
dc.contributor.departmentBIOMED INST FOR GLOBAL HEALTH RES & TECH
dc.contributor.departmentBIOMEDICAL ENGINEERING
dc.description.doi10.1038/s42003-021-01793-z
dc.description.sourcetitleCommunications Biology
dc.description.volume4
dc.description.issue1
dc.description.page271
dc.published.statePublished
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