MICROFLUIDIC DETECTION OF BACTERIAL INFECTION

Abstract

Sepsis is a serious disease due to microbial infection, so it is vital to separate the pathogen as early as possible. Separation technologies can be combined with microfluidics to achieve high sensitivity. Microparticles can be inertially focused or undergo Dean migration inside the microchannel, and spiral microfluidic chips can be designed for pathogen detection. Traditional devices employing inertial focusing have low throughput. To improve it, spiral microfluidic chips can enable various particles to undergo Dean migration for bacterial separation. This thesis aims to explore the bacterial separation process based on Dean migration and spiral microfluidic chip. In this thesis, the separation principle, design and fabrication were proposed, and the recovery rate of bacteria, the removal rate of blood cells were evaluated through different methods. The goal is to develop a spiral microfluidic device for bacterial separation from blood for clinical application.