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dc.titleDesigning and constructing a phage display synthesized single domain antibodies library based on camel VHHs frame for screening and identifying humanized TNF-?-specific nanobody
dc.contributor.authorNie, Jifan
dc.contributor.authorMa, Xingyuan
dc.contributor.authorHu, Fabiao
dc.contributor.authorMiao, Hui
dc.contributor.authorFeng, Xin
dc.contributor.authorZhang, Peiwen
dc.contributor.authorHan, Myong Hun
dc.contributor.authorYou, Fang
dc.contributor.authorYang, Yi
dc.contributor.authorZhang, Wenlian
dc.contributor.authorZheng, Wenyun
dc.identifier.citationNie, Jifan, Ma, Xingyuan, Hu, Fabiao, Miao, Hui, Feng, Xin, Zhang, Peiwen, Han, Myong Hun, You, Fang, Yang, Yi, Zhang, Wenlian, Zheng, Wenyun (2021-05-01). Designing and constructing a phage display synthesized single domain antibodies library based on camel VHHs frame for screening and identifying humanized TNF-?-specific nanobody. Biomedicine and Pharmacotherapy 137 : 111328. ScholarBank@NUS Repository.
dc.description.abstractTumor necrosis factor (TNF-?) is an important clinically tested cytokine that could induce autoimmune diseases and inflammation. Therefore, the anti-TNF-? therapy strategy was developed and used therapeutically in various diseases, especially in the cytokine storm associated chimeric antigen receptor (CAR) T-cell therapy and antiviral therapy. Compare with other anti-TNF-? inhibitors, anti-TNF-? Nb (nanobody) has many unique advantages. Herein, we reported a novel humanized scaffold for library construction, which could be soluble and expressed in Escherichia coli (E.coli), and the efficiency capacity could reach as high as 2.01 × 109. Meanwhile, an anti-TNF-? Nb was selected for further study after 4 rounds of screening, NT-3, as the optimal Nb could effectively inhibit TNF-mediated cytotoxicity. The IC50 of NT-3 was determined as 0.804 ?M, and its apoptosis inhibition rate was 62.47 % in L929 cells. Furthermore, the molecular docking results showed that complementarity-determining regions (CDRs) of NT-3 could connect to TNF for blocking function through strong hydrogen bonds and salt bridges. In general, our study not only provided a good Nb screening platform in vitro without animal immunization, but also generated a series of novel humanized anti-TNF-? Nb candidates with potential applications. © 2021
dc.publisherElsevier Masson s.r.l.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.sourceScopus OA2021
dc.subjectDesigning and constructing
dc.subjectHumanized TNF-?-specific Nb
dc.subjectPhage display library
dc.subjectScreening and identifyin
dc.subjectSingle domain antibodies
dc.description.sourcetitleBiomedicine and Pharmacotherapy
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