Please use this identifier to cite or link to this item: https://doi.org/10.1523/eneuro.0079-21.2021
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dc.titleCharacterization of seizure induction methods in drosophila
dc.contributor.authorMituzaite, J.
dc.contributor.authorPetersen, R.
dc.contributor.authorClaridge-Chang, A.
dc.contributor.authorBaines, R.A.
dc.date.accessioned2022-10-12T08:11:13Z
dc.date.available2022-10-12T08:11:13Z
dc.date.issued2021-07-01
dc.identifier.citationMituzaite, J., Petersen, R., Claridge-Chang, A., Baines, R.A. (2021-07-01). Characterization of seizure induction methods in drosophila. eNeuro 8 (4) : ENEURO.0079-21.2021. ScholarBank@NUS Repository. https://doi.org/10.1523/eneuro.0079-21.2021
dc.identifier.issn2373-2822
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/232535
dc.description.abstractEpilepsy is one of the most common neurologic disorders. Around one third of patients do not respond to current medications. This lack of treatment indicates a need for better understanding of the underlying mechanisms and, importantly, the identification of novel targets for drug manipulation. The fruit fly Drosophila melanogaster has a fast reproduction time, powerful genetics, and facilitates large sample sizes, making it a strong model of seizure mechanisms. To better understand behavioral and physiological phenotypes across major fly seizure genotypes we systematically measured seizure severity and secondary behavioral pheno-types at both the larval and adult stage. Comparison of several seizure-induction methods; specifically electri-cal, mechanical and heat induction, show that larval electroshock is the most effective at inducing seizures across a wide range of seizure-prone mutants tested. Locomotion in adults and larvae was found to be non-predictive of seizure susceptibility. Recording activity in identified larval motor neurons revealed variations in action potential (AP) patterns, across different genotypes, but these patterns did not correlate with seizure susceptibility. To conclude, while there is wide variation in mechanical induction, heat induction, and secondary phenotypes, electroshock is the most consistent method of seizure induction across known major seizure genotypes in Drosophila. © 2021 Mituzaite et al.
dc.publisherSociety for Neuroscience
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceScopus OA2021
dc.subjectAntiepileptic
dc.subjectDrosophila
dc.subjectEpilepsy
dc.subjectInsect
dc.subjectModel
dc.subjectSeizure induction
dc.typeArticle
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.description.doi10.1523/eneuro.0079-21.2021
dc.description.sourcetitleeNeuro
dc.description.volume8
dc.description.issue4
dc.description.pageENEURO.0079-21.2021
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