HIGH THROUGHPUT ANTIBODY FUNCTIONAL ASSAYS VIA DROPLET MICROFLUIDICS

Abstract

In recent years, therapeutics based on monoclonal antibodies (mAbs) have been gaining traction as one of the most promising tools in the fight against numerous diseases. To discover new mAb candidates, large populations of antibody-secreting cells (ASCs) can be screened using high-throughput techniques such as flow cytometry and phage display. However, current high-throughput screening methods typically rely on antibody (Ab) binding affinity as a proxy for mAbs functional efficacy. Herein, to bridge the abovementioned research gap, an integrated droplet microfluidic platform capable of functional screening of ASCs at a high throughput was established.