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Title: Oxidative and Nitrosative Stress and Histone Deacetylase-2 Activity in Exacerbations of COPD
Authors: Footitt, Joseph
Mallia, Patrick
Durham, Andrew L
Ho, W Eugene 
Trujillo-Torralbo, Maria-Belen
Telcian, Aurica G
Del Rosario, Ajerico
Chang, Cheng 
Peh, Hong-Yong 
Kebadze, Tatiana
Aniscenko, Julia
Stanciu, Luminita
Essilfie-Quaye, Sarah
Ito, Kazuhiro
Barnes, Peter J
Elkin, Sarah L
Kon, Onn M
Wong, WS Fred 
Adcock, Ian M
Johnston, Sebastian L
Keywords: Science & Technology
Life Sciences & Biomedicine
Critical Care Medicine
Respiratory System
General & Internal Medicine
host defense
viral disease
Issue Date: 1-Jan-2016
Citation: Footitt, Joseph, Mallia, Patrick, Durham, Andrew L, Ho, W Eugene, Trujillo-Torralbo, Maria-Belen, Telcian, Aurica G, Del Rosario, Ajerico, Chang, Cheng, Peh, Hong-Yong, Kebadze, Tatiana, Aniscenko, Julia, Stanciu, Luminita, Essilfie-Quaye, Sarah, Ito, Kazuhiro, Barnes, Peter J, Elkin, Sarah L, Kon, Onn M, Wong, WS Fred, Adcock, Ian M, Johnston, Sebastian L (2016-01-01). Oxidative and Nitrosative Stress and Histone Deacetylase-2 Activity in Exacerbations of COPD. CHEST 149 (1) : 62-73. ScholarBank@NUS Repository.
Abstract: BACKGROUND: Respiratory virus infections are commonly associated with COPD exacerbations, but little is known about the mechanisms linking virus infection to exacerbations. Pathogenic mechanisms in stable COPD include oxidative and nitrosative stress and reduced activity of histone deacetylase-2 (HDAC2), but their roles in COPD exacerbations is unknown. We investigated oxidative and nitrosative stress (O&NS) and HDAC2 in COPD exacerbations using experimental rhinovirus infection. METHODS: Nine subjects with COPD (Global Initiative for Chronic Obstructive Lung Disease stage II), 10 smokers, and 11 nonsmokers were successfully infected with rhinovirus. Markers of O&NS-Associated cellular damage, and inflammatory mediators and proteases were measured in sputum, and HDAC2 activity was measured in sputum and bronchoalveolar macrophages. In an in vitro model, monocyte-derived THP-1 cells were infected with rhinovirus and nitrosylation and activity of HDAC2 was measured. RESULTS: Rhinovirus infection induced significant increases in airways inflammation and markers of O&NS in subjects with COPD. O&NS markers correlated with virus load and inflammatory markers. Macrophage HDAC2 activity was reduced during exacerbation and correlated inversely with virus load, inflammatory markers, and nitrosative stress. Sputum macrophage HDAC2 activity pre-infection was inversely associated with sputum virus load and inflammatory markers during exacerbation. Rhinovirus infection of monocytes induced nitrosylation of HDAC2 and reduced HDAC2 activity; inhibition of O&NS inhibited rhinovirus-induced inflammatory cytokines. CONCLUSIONS: O&NS, airways inflammation, and impaired HDAC2 may be important mechanisms of virus-induced COPD exacerbations. Therapies targeting these mechanisms offer potential new treatments for COPD exacerbations. Copyright © 2016 American College of Chest Physicians. Published by Elsevier Inc. All rights reserved.
Source Title: CHEST
ISSN: 0012-3692,1931-3543
DOI: 10.1378/chest.14-2637
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