Please use this identifier to cite or link to this item:
https://doi.org/10.1002/acg2.101
DC Field | Value | |
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dc.title | Rapid production of clinical-grade SARS-CoV-2 specific T cells. | |
dc.contributor.author | Leung, Wing | |
dc.contributor.author | Soh, Teck Guan | |
dc.contributor.author | Linn, Yeh Ching | |
dc.contributor.author | Low, Jenny Guek-Hong | |
dc.contributor.author | Loh, Jiashen | |
dc.contributor.author | Chan, Marieta | |
dc.contributor.author | Chng, Wee Joo | |
dc.contributor.author | Koh, Liang Piu | |
dc.contributor.author | Poon, Michelle Li-Mei | |
dc.contributor.author | Ng, King Pan | |
dc.contributor.author | Kuick, Chik Hong | |
dc.contributor.author | Tan, Thuan Tong | |
dc.contributor.author | Tan, Lip Kun | |
dc.contributor.author | Seng, Michaela Su-Fern | |
dc.date.accessioned | 2022-07-18T06:23:27Z | |
dc.date.available | 2022-07-18T06:23:27Z | |
dc.date.issued | 2020-10 | |
dc.identifier.citation | Leung, Wing, Soh, Teck Guan, Linn, Yeh Ching, Low, Jenny Guek-Hong, Loh, Jiashen, Chan, Marieta, Chng, Wee Joo, Koh, Liang Piu, Poon, Michelle Li-Mei, Ng, King Pan, Kuick, Chik Hong, Tan, Thuan Tong, Tan, Lip Kun, Seng, Michaela Su-Fern (2020-10). Rapid production of clinical-grade SARS-CoV-2 specific T cells.. Adv Cell Gene Ther 3 (4) : e101-. ScholarBank@NUS Repository. https://doi.org/10.1002/acg2.101 | |
dc.identifier.issn | 2573-8461 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/228757 | |
dc.description.abstract | Objectives: To determine whether the frequencies of SARS-CoV-2-specific T cells are sufficiently high in the blood of convalescent donors and whether it is technically feasible to manufacture clinical-grade products overnight for T-cell therapy and assessment of COVID-19 immunity. Methods: One unit of whole blood or leukapheresis was collected from each donor following standard blood bank practices. The leukocytes were stimulated using overlapping peptides of SARS-CoV-2, covering the immunodominant sequence domains of the S protein and the complete sequence of the N and M proteins. Thereafter, functionally reactive cells were enriched overnight using an automated device capturing IFNγ-secreting cells. Results: From 1 × 109 leukocytes, a median of 0.98 × 106 (range 0.56-2.95) IFNγ + T cells were produced from each of the six donors, suggesting a high frequency of SARS-CoV-2 reactive T cells in their blood, even though only one donor had severe COVID-19 requiring mechanical ventilation whereas the other five donors had minor symptoms. A median of 57% of the enriched T cells were IFNγ+ (range 20%-74%), with preferential enrichment of CD56+ T cells and effector memory T cells. TCRVβ-spectratyping confirmed distinctively tall oligoclonal peaks in final products. With just six donors, the probability that a recipient would share at least one HLA allele with one of the donors is >88% among Caucasian, >95% among Chinese, >97% among Malay, and >99% among Indian populations. Conclusions: High frequencies of rapid antigen-reactive T cells were found in convalescent donors, regardless of severity of COVID-19. The feasibility of clinical-grade production of SARS-CoV-2-specific T cells overnight for therapeutics and diagnostics is revealed. | |
dc.publisher | Hindawi Limited | |
dc.source | Elements | |
dc.subject | COVID‐19 | |
dc.subject | SARS‐CoV‐2 | |
dc.subject | T Cells | |
dc.subject | adoptive cell therapy | |
dc.type | Article | |
dc.date.updated | 2022-07-14T03:46:02Z | |
dc.contributor.department | CANCER SCIENCE INSTITUTE OF SINGAPORE | |
dc.contributor.department | DEAN'S OFFICE (DUKE-NUS MEDICAL SCHOOL) | |
dc.contributor.department | MEDICINE | |
dc.contributor.department | PATHOLOGY | |
dc.contributor.department | DUKE-NUS MEDICAL SCHOOL | |
dc.description.doi | 10.1002/acg2.101 | |
dc.description.sourcetitle | Adv Cell Gene Ther | |
dc.description.volume | 3 | |
dc.description.issue | 4 | |
dc.description.page | e101- | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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Rapid production of clinical-grade SARS-CoV-2 specific T cells.pdf | Published version | 756.87 kB | Adobe PDF | OPEN | Published | View/Download |
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