Please use this identifier to cite or link to this item:
https://doi.org/10.3390/pr9040629
DC Field | Value | |
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dc.title | High Throughput Expression Screening of Arabinofuranosyltransferases from Mycobacteria | |
dc.contributor.author | Rodrigues, Jose | |
dc.contributor.author | Almeida, Vanessa T | |
dc.contributor.author | Rosario, Ana L | |
dc.contributor.author | Tan, Yong Zi | |
dc.contributor.author | Kloss, Brian | |
dc.contributor.author | Mancia, Filippo | |
dc.contributor.author | Archer, Margarida | |
dc.date.accessioned | 2022-06-20T03:39:40Z | |
dc.date.available | 2022-06-20T03:39:40Z | |
dc.date.issued | 2021-04-01 | |
dc.identifier.citation | Rodrigues, Jose, Almeida, Vanessa T, Rosario, Ana L, Tan, Yong Zi, Kloss, Brian, Mancia, Filippo, Archer, Margarida (2021-04-01). High Throughput Expression Screening of Arabinofuranosyltransferases from Mycobacteria. PROCESSES 9 (4). ScholarBank@NUS Repository. https://doi.org/10.3390/pr9040629 | |
dc.identifier.issn | 22279717 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/227198 | |
dc.description.abstract | Studies on membrane proteins can help to develop new drug targets and treatments for a variety of diseases. However, membrane proteins continue to be among the most challenging targets in structural biology. This uphill endeavor can be even harder for membrane proteins from Mycobacterium species, which are notoriously difficult to express in heterologous systems. Arabino-furanosyltransferases are involved in mycobacterial cell wall synthesis and thus potential targets for antituberculosis drugs. A set of 96 mycobacterial genes coding for Arabinofuranosyltransferases was selected, of which 17 were successfully expressed in E. coli and purified by metal-affinity chro-matography. We herein present an efficient high-throughput strategy to screen in microplates a large number of targets from Mycobacteria and select the best conditions for large-scale protein production to pursue functional and structural studies. This methodology can be applied to other targets, is cost and time effective and can be implemented in common laboratories. | |
dc.language.iso | en | |
dc.publisher | MDPI | |
dc.source | Elements | |
dc.subject | Science & Technology | |
dc.subject | Technology | |
dc.subject | Engineering, Chemical | |
dc.subject | Engineering | |
dc.subject | membrane proteins | |
dc.subject | overexpression in E. coli | |
dc.subject | protein purification | |
dc.subject | high-throughput protocol | |
dc.subject | Arabinofuranosyltransferases | |
dc.subject | Mycobacteria | |
dc.subject | MEMBRANE-PROTEIN OVEREXPRESSION | |
dc.subject | ESCHERICHIA-COLI | |
dc.subject | BIOSYNTHESIS | |
dc.subject | ARABINOGALACTAN | |
dc.subject | PURIFICATION | |
dc.subject | STRATEGY | |
dc.type | Article | |
dc.date.updated | 2022-06-18T14:50:05Z | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.doi | 10.3390/pr9040629 | |
dc.description.sourcetitle | PROCESSES | |
dc.description.volume | 9 | |
dc.description.issue | 4 | |
dc.published.state | Published | |
Appears in Collections: | Elements Staff Publications |
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File | Description | Size | Format | Access Settings | Version | |
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2021-04-02_Rodrigues_Processes.pdf | Published version | 1.89 MB | Adobe PDF | OPEN | None | View/Download |
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