Please use this identifier to cite or link to this item: https://doi.org/10.25540/XT13-8VRZ
Title: Surface curvature and basal hydraulic stress induce spatial bias in cell extrusion
Creators: CHENG-KUANG HUANG
YONG XIANBIN
TAI WAI DAVID SHE
CHWEE TECK LIM
NUS Contact: 
Subject: Biophysics, Cell biology, Bioengineering, Biomaterials
DOI: doi:10.25540/XT13-8VRZ
Description: 

Reflection interference contrast analysis; pFAK/FAK analysis; and osmolarity calculations

Citation: CHENG-KUANG HUANG, YONG XIANBIN, TAI WAI DAVID SHE, CHWEE TECK LIM (2022-04-25). Surface curvature and basal hydraulic stress induce spatial bias in cell extrusion. ScholarBank@NUS Repository. [Dataset]. https://doi.org/10.25540/XT13-8VRZ
License: Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
Appears in Collections:Staff Dataset

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File Description SizeFormatAccess Settings 
histogramHillValley.7zR code and processed image data for RICM of curved surfaces980.27 kBUnknown

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osmolarityCalcu.xlsxcalculation of media osmolarity14.54 kBMicrosoft Excel XML

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Processed FAK data.7zR code and processed image data for pFAK FAK immunocytochemistry over curved surfaces110.36 MBUnknown

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histogramTime.7zR code and processed image data for RICM of hyper- to iso-Osmotic treatment over time397.65 MBUnknown

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substrateDimensions.xlsxWave substrate dimension characterization19.36 kBMicrosoft Excel XML

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3DTFM.zipCodes used to estimate the 3D forces exerted by the cells via a finite element method3.86 MBZIP

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3DTFM_analysis.zipCodes used to analysis the 3D forces exerted by the cells on the cylindrical wave substrates. Also contains the data showing the processed forces at each node1.65 MBZIP

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Extrusions.zipCodes used to calculate the normalized extrusion rate from the detected extrusion events. Also contains the data for the normalized extrusion rates as well as the extrusion counts for each condition965.49 kBZIP

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opticalflow3D.zipSource code for 3D Farneback algorithm that was used to obtain the displacement between the two bead image volumes115.69 kBZIP

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Unet-extrusion-detection.zipSource code for the deeply supervised attention-gated residual U-net that was trained to detect extrusion events1.3 MBZIP

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westernBlotMaterialsand Analysis.docxAntibodies, devices, and software used in FAK and Akt immunoblotting, and the statistical analysis of the blots18.92 kBMicrosoft Word XML

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This item is licensed under a Creative Commons License Creative Commons