Please use this identifier to cite or link to this item:
https://doi.org/10.25540/XT13-8VRZ
| Title: | Surface curvature and basal hydraulic stress induce spatial bias in cell extrusion | Creators: | CHENG-KUANG HUANG YONG XIANBIN TAI WAI DAVID SHE CHWEE TECK LIM |
NUS Contact: | Subject: | Biophysics, Cell biology, Bioengineering, Biomaterials | DOI: | doi:10.25540/XT13-8VRZ | Description: | Reflection interference contrast analysis; pFAK/FAK analysis; and osmolarity calculations |
Citation: | CHENG-KUANG HUANG, YONG XIANBIN, TAI WAI DAVID SHE, CHWEE TECK LIM (2022-04-25). Surface curvature and basal hydraulic stress induce spatial bias in cell extrusion. ScholarBank@NUS Repository. [Dataset]. https://doi.org/10.25540/XT13-8VRZ | License: | Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ |
| Appears in Collections: | Staff Dataset |
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Files in This Item:
| File | Description | Size | Format | Access Settings | |
|---|---|---|---|---|---|
| histogramHillValley.7z | R code and processed image data for RICM of curved surfaces | 980.27 kB | Unknown | CLOSED | Request a copy |
| osmolarityCalcu.xlsx | calculation of media osmolarity | 14.54 kB | Microsoft Excel XML | OPEN | View/Download |
| Processed FAK data.7z | R code and processed image data for pFAK FAK immunocytochemistry over curved surfaces | 110.36 MB | Unknown | OPEN | View/Download |
| histogramTime.7z | R code and processed image data for RICM of hyper- to iso-Osmotic treatment over time | 397.65 MB | Unknown | OPEN | View/Download |
| substrateDimensions.xlsx | Wave substrate dimension characterization | 19.36 kB | Microsoft Excel XML | OPEN | View/Download |
| 3DTFM.zip | Codes used to estimate the 3D forces exerted by the cells via a finite element method | 3.86 MB | ZIP | OPEN | View/Download |
| 3DTFM_analysis.zip | Codes used to analysis the 3D forces exerted by the cells on the cylindrical wave substrates. Also contains the data showing the processed forces at each node | 1.65 MB | ZIP | OPEN | View/Download |
| Extrusions.zip | Codes used to calculate the normalized extrusion rate from the detected extrusion events. Also contains the data for the normalized extrusion rates as well as the extrusion counts for each condition | 965.49 kB | ZIP | OPEN | View/Download |
| opticalflow3D.zip | Source code for 3D Farneback algorithm that was used to obtain the displacement between the two bead image volumes | 115.69 kB | ZIP | OPEN | View/Download |
| Unet-extrusion-detection.zip | Source code for the deeply supervised attention-gated residual U-net that was trained to detect extrusion events | 1.3 MB | ZIP | OPEN | View/Download |
| westernBlotMaterialsand Analysis.docx | Antibodies, devices, and software used in FAK and Akt immunoblotting, and the statistical analysis of the blots | 18.92 kB | Microsoft Word XML | OPEN | View/Download |
| NcleiSeg.zip | R code and segmented image data of nuclei on 50, 100, and 200 um hills and valleys | 59.39 MB | ZIP | OPEN | View/Download |
| RICM_DataPlusAnalysis.zip | R code and processed image data for RICM of 100 and 200 um hills and valleys | 6.88 MB | ZIP | OPEN | View/Download |
This item is licensed under a Creative Commons License
