Please use this identifier to cite or link to this item:
https://doi.org/10.5483/BMBRep.2017.50.5.044
DC Field | Value | |
---|---|---|
dc.title | CRISPR system for genome engineering: the application for autophagy study | |
dc.contributor.author | Cui, Jianzhou | |
dc.contributor.author | Chew, Shirley Jia Li | |
dc.contributor.author | Shi, Yin | |
dc.contributor.author | Gong, Zhiyuan | |
dc.contributor.author | Shen, Han-Ming | |
dc.date.accessioned | 2022-04-25T01:10:22Z | |
dc.date.available | 2022-04-25T01:10:22Z | |
dc.date.issued | 2017-05-31 | |
dc.identifier.citation | Cui, Jianzhou, Chew, Shirley Jia Li, Shi, Yin, Gong, Zhiyuan, Shen, Han-Ming (2017-05-31). CRISPR system for genome engineering: the application for autophagy study. BMB REPORTS 50 (5) : 247-256. ScholarBank@NUS Repository. https://doi.org/10.5483/BMBRep.2017.50.5.044 | |
dc.identifier.issn | 19766696 | |
dc.identifier.issn | 1976670X | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/224158 | |
dc.description.abstract | CRISPR/Cas9 is the latest tool introduced in the field of genome engineering and is so far the best genome-editing tool as compared to its precedents such as, meganucleases, zinc finger nucleases (ZFNs) and transcription activator-like effectors (TALENs). The simple design and assembly of the CRISPR/Cas9 system makes genome editing easy to perform as it uses small guide RNAs that correspond to their DNA targets for high efficiency editing. This has helped open the doors for multiplexible genome targeting in many species that were intractable using old genetic perturbation techniques. Currently, The CRISPR system is revolutionizing the way biological researches are conducted and paves a bright future not only in research but also in medicine and biotechnology. In this review, we evaluated the history, types and structure, the mechanism of action of CRISPR/Cas System. In particular, we focused on the application of this powerful tool in autophagy research. | |
dc.language.iso | en | |
dc.publisher | KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY | |
dc.source | Elements | |
dc.subject | Science & Technology | |
dc.subject | Life Sciences & Biomedicine | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | Autophagy | |
dc.subject | CRISPR/Cas9 | |
dc.subject | gRNA | |
dc.subject | Off-target | |
dc.subject | PAM | |
dc.subject | RNA-GUIDED ENDONUCLEASE | |
dc.subject | ZINC-FINGER NUCLEASES | |
dc.subject | CAS SYSTEM | |
dc.subject | CAENORHABDITIS-ELEGANS | |
dc.subject | TARGETED MUTAGENESIS | |
dc.subject | HUMAN-CELLS | |
dc.subject | HOMOLOGOUS RECOMBINATION | |
dc.subject | ADAPTIVE IMMUNITY | |
dc.subject | GENE-EXPRESSION | |
dc.subject | DNA | |
dc.type | Review | |
dc.date.updated | 2022-04-24T09:00:13Z | |
dc.contributor.department | DEAN'S OFFICE (MEDICINE) | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.contributor.department | PHYSIOLOGY | |
dc.description.doi | 10.5483/BMBRep.2017.50.5.044 | |
dc.description.sourcetitle | BMB REPORTS | |
dc.description.volume | 50 | |
dc.description.issue | 5 | |
dc.description.page | 247-256 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
Show simple item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
CRISPR system for genome engineering the application for autophagy study.pdf | Published version | 1.83 MB | Adobe PDF | OPEN | Published | View/Download |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.