Please use this identifier to cite or link to this item:
https://doi.org/10.1093/nar/gkw1143
DC Field | Value | |
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dc.title | Enhanced non-viral gene delivery by coordinated endosomal release and inhibition of beta-tubulin deactylase | |
dc.contributor.author | Ho, Yoon Khei | |
dc.contributor.author | Zhou, Li Han | |
dc.contributor.author | Tam, Kam C | |
dc.contributor.author | Too, Heng Phon | |
dc.date.accessioned | 2022-04-19T06:59:04Z | |
dc.date.available | 2022-04-19T06:59:04Z | |
dc.date.issued | 2017-04-07 | |
dc.identifier.citation | Ho, Yoon Khei, Zhou, Li Han, Tam, Kam C, Too, Heng Phon (2017-04-07). Enhanced non-viral gene delivery by coordinated endosomal release and inhibition of beta-tubulin deactylase. NUCLEIC ACIDS RESEARCH 45 (6). ScholarBank@NUS Repository. https://doi.org/10.1093/nar/gkw1143 | |
dc.identifier.issn | 03051048 | |
dc.identifier.issn | 13624962 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/219298 | |
dc.description.abstract | Efficient non-viral gene delivery is highly desirable but often unattainable with some cell-types. We report here that non-viral DNA polyplexes can efficiently transfect differentiated neuronal and stem cells. Polyplex transfection centrifugation protocols was enhanced by including a simultaneous treatment with a DOPE/CHEMS lipid suspension and a microtubule inhibitor, Tubastatin A. Lipoplex transfection protocols were not improved by this treatment. This mechanism of action was unravelled by systematically identifying and rationally mitigating barriers limiting high transfection efficiency, allowing unexpected improvements in the transfection of mesenchymal stem cells (MSC), primary neuron and several hard-to-transfect cell types beyond what are currently achievable using cationic polymers. The optimized formulation and method achieved high transfection efficiency with no adverse effects on cell viability, cell proliferation or differentiation. High efficiency modification of MSC for cytokine overexpression, efficient generation of dopaminergic neuron using neural stem cells and enhanced genome editing with CRISPR-Cas9 were demonstrated. In summary, this study described a cost-effective method for efficient, rapid and scalable workflow for ex vivo gene delivery using a myriad of nucleic acids including plasmid DNA, mRNA, siRNA and shRNA. | |
dc.language.iso | en | |
dc.publisher | OXFORD UNIV PRESS | |
dc.source | Elements | |
dc.subject | Science & Technology | |
dc.subject | Life Sciences & Biomedicine | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | MESENCHYMAL STEM-CELLS | |
dc.subject | INTRACELLULAR TRAFFICKING | |
dc.subject | DOPAMINERGIC-NEURONS | |
dc.subject | TRANSFECTION | |
dc.subject | PH | |
dc.subject | POLYPLEXES | |
dc.subject | EXPRESSION | |
dc.subject | EFFICIENCY | |
dc.subject | TRANSPORT | |
dc.subject | AGENTS | |
dc.type | Article | |
dc.date.updated | 2022-04-19T00:35:58Z | |
dc.contributor.department | BIOCHEMISTRY | |
dc.description.doi | 10.1093/nar/gkw1143 | |
dc.description.sourcetitle | NUCLEIC ACIDS RESEARCH | |
dc.description.volume | 45 | |
dc.description.issue | 6 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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File | Description | Size | Format | Access Settings | Version | |
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Enhanced non-viral gene delivery by coordinated endosomal release and inhibition of β-tubulin deactylase.pdf | Published version | 4.32 MB | Adobe PDF | OPEN | None | View/Download |
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