Please use this identifier to cite or link to this item: https://doi.org/10.1093/stmcls/sxab009
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dc.titleA Runx1-enhancer Element eR1 Identified Lineage Restricted Mammary Luminal Stem Cells
dc.contributor.authorMatsuo, Junichi
dc.contributor.authorMon, Naing Naing
dc.contributor.authorDouchi, Daisuke
dc.contributor.authorYamamura, Akihiro
dc.contributor.authorKulkarni, Madhura
dc.contributor.authorHeng, Dede Liana
dc.contributor.authorChen, Sabirah
dc.contributor.authorNuttonmanit, Napat
dc.contributor.authorLi, Ying
dc.contributor.authorYang, Henry
dc.contributor.authorLee, May Yin
dc.contributor.authorTam, Wai Leong
dc.contributor.authorOsato, Motomi
dc.contributor.authorChuang, Linda Shyue Huey
dc.contributor.authorIto, Yoshiaki
dc.date.accessioned2022-04-13T02:28:49Z
dc.date.available2022-04-13T02:28:49Z
dc.date.issued2022-03-03
dc.identifier.citationMatsuo, Junichi, Mon, Naing Naing, Douchi, Daisuke, Yamamura, Akihiro, Kulkarni, Madhura, Heng, Dede Liana, Chen, Sabirah, Nuttonmanit, Napat, Li, Ying, Yang, Henry, Lee, May Yin, Tam, Wai Leong, Osato, Motomi, Chuang, Linda Shyue Huey, Ito, Yoshiaki (2022-03-03). A Runx1-enhancer Element eR1 Identified Lineage Restricted Mammary Luminal Stem Cells. STEM CELLS 40 (1) : 112-122. ScholarBank@NUS Repository. https://doi.org/10.1093/stmcls/sxab009
dc.identifier.issn1066-5099
dc.identifier.issn1549-4918
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/219076
dc.description.abstract<jats:title>Abstract</jats:title> <jats:p>Mammary gland homeostasis is maintained by adult tissue stem-progenitor cells residing within the luminal and basal epithelia. Dysregulation of mammary stem cells is a key mechanism for cancer development. However, stem cell characterization is challenging because reporter models using cell-specific promoters do not fully recapitulate the mammary stem cell populations. We previously found that a 270-basepair Runx1 enhancer element, named eR1, marked stem cells in the blood and stomach. Here, we identified eR1 activity in a rare subpopulation of the ERα-negative luminal epithelium in mouse mammary glands. Lineage-tracing using an eR1-CreERT2 mouse model revealed that eR1+ luminal cells generated the entire luminal lineage and milk-secreting alveoli—eR1 therefore specifically marks lineage-restricted luminal stem cells. eR1-targeted-conditional knockout of Runx1 led to the expansion of luminal epithelial cells, accompanied by elevated ERα expression. Our findings demonstrate a definitive role for Runx1 in the regulation of the eR1-positive luminal stem cell proliferation during mammary homeostasis. Our findings identify a mechanistic link for Runx1 in stem cell proliferation and its dysregulation in breast cancer. Runx1 inactivation is therefore likely to be an early hit in the cell-of-origin of ERα+ luminal type breast cancer.</jats:p>
dc.language.isoen
dc.publisherOXFORD UNIV PRESS
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectCell & Tissue Engineering
dc.subjectBiotechnology & Applied Microbiology
dc.subjectOncology
dc.subjectCell Biology
dc.subjectHematology
dc.subjectRunx1
dc.subjectenhancer
dc.subjecteR1
dc.subjectmammary gland
dc.subjectstem cell
dc.subjectER alpha
dc.subjectBREAST-CANCER
dc.subjectFATE
dc.subjectREGENERATION
dc.subjectRUNX1
dc.typeArticle
dc.date.updated2022-04-13T02:08:33Z
dc.contributor.departmentCANCER SCIENCE INSTITUTE OF SINGAPORE
dc.contributor.departmentDEPT OF MEDICINE
dc.contributor.departmentDEPT OF BIOCHEMISTRY
dc.description.doi10.1093/stmcls/sxab009
dc.description.sourcetitleSTEM CELLS
dc.description.volume40
dc.description.issue1
dc.description.page112-122
dc.published.statePublished
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