Please use this identifier to cite or link to this item:
https://doi.org/10.1093/stmcls/sxab009
DC Field | Value | |
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dc.title | A Runx1-enhancer Element eR1 Identified Lineage Restricted Mammary Luminal Stem Cells | |
dc.contributor.author | Matsuo, Junichi | |
dc.contributor.author | Mon, Naing Naing | |
dc.contributor.author | Douchi, Daisuke | |
dc.contributor.author | Yamamura, Akihiro | |
dc.contributor.author | Kulkarni, Madhura | |
dc.contributor.author | Heng, Dede Liana | |
dc.contributor.author | Chen, Sabirah | |
dc.contributor.author | Nuttonmanit, Napat | |
dc.contributor.author | Li, Ying | |
dc.contributor.author | Yang, Henry | |
dc.contributor.author | Lee, May Yin | |
dc.contributor.author | Tam, Wai Leong | |
dc.contributor.author | Osato, Motomi | |
dc.contributor.author | Chuang, Linda Shyue Huey | |
dc.contributor.author | Ito, Yoshiaki | |
dc.date.accessioned | 2022-04-13T02:28:49Z | |
dc.date.available | 2022-04-13T02:28:49Z | |
dc.date.issued | 2022-03-03 | |
dc.identifier.citation | Matsuo, Junichi, Mon, Naing Naing, Douchi, Daisuke, Yamamura, Akihiro, Kulkarni, Madhura, Heng, Dede Liana, Chen, Sabirah, Nuttonmanit, Napat, Li, Ying, Yang, Henry, Lee, May Yin, Tam, Wai Leong, Osato, Motomi, Chuang, Linda Shyue Huey, Ito, Yoshiaki (2022-03-03). A Runx1-enhancer Element eR1 Identified Lineage Restricted Mammary Luminal Stem Cells. STEM CELLS 40 (1) : 112-122. ScholarBank@NUS Repository. https://doi.org/10.1093/stmcls/sxab009 | |
dc.identifier.issn | 1066-5099 | |
dc.identifier.issn | 1549-4918 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/219076 | |
dc.description.abstract | <jats:title>Abstract</jats:title> <jats:p>Mammary gland homeostasis is maintained by adult tissue stem-progenitor cells residing within the luminal and basal epithelia. Dysregulation of mammary stem cells is a key mechanism for cancer development. However, stem cell characterization is challenging because reporter models using cell-specific promoters do not fully recapitulate the mammary stem cell populations. We previously found that a 270-basepair Runx1 enhancer element, named eR1, marked stem cells in the blood and stomach. Here, we identified eR1 activity in a rare subpopulation of the ERα-negative luminal epithelium in mouse mammary glands. Lineage-tracing using an eR1-CreERT2 mouse model revealed that eR1+ luminal cells generated the entire luminal lineage and milk-secreting alveoli—eR1 therefore specifically marks lineage-restricted luminal stem cells. eR1-targeted-conditional knockout of Runx1 led to the expansion of luminal epithelial cells, accompanied by elevated ERα expression. Our findings demonstrate a definitive role for Runx1 in the regulation of the eR1-positive luminal stem cell proliferation during mammary homeostasis. Our findings identify a mechanistic link for Runx1 in stem cell proliferation and its dysregulation in breast cancer. Runx1 inactivation is therefore likely to be an early hit in the cell-of-origin of ERα+ luminal type breast cancer.</jats:p> | |
dc.language.iso | en | |
dc.publisher | OXFORD UNIV PRESS | |
dc.source | Elements | |
dc.subject | Science & Technology | |
dc.subject | Life Sciences & Biomedicine | |
dc.subject | Cell & Tissue Engineering | |
dc.subject | Biotechnology & Applied Microbiology | |
dc.subject | Oncology | |
dc.subject | Cell Biology | |
dc.subject | Hematology | |
dc.subject | Runx1 | |
dc.subject | enhancer | |
dc.subject | eR1 | |
dc.subject | mammary gland | |
dc.subject | stem cell | |
dc.subject | ER alpha | |
dc.subject | BREAST-CANCER | |
dc.subject | FATE | |
dc.subject | REGENERATION | |
dc.subject | RUNX1 | |
dc.type | Article | |
dc.date.updated | 2022-04-13T02:08:33Z | |
dc.contributor.department | CANCER SCIENCE INSTITUTE OF SINGAPORE | |
dc.contributor.department | MEDICINE | |
dc.contributor.department | BIOCHEMISTRY | |
dc.description.doi | 10.1093/stmcls/sxab009 | |
dc.description.sourcetitle | STEM CELLS | |
dc.description.volume | 40 | |
dc.description.issue | 1 | |
dc.description.page | 112-122 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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sxab009.pdf | 25.85 MB | Adobe PDF | OPEN | None | View/Download |
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