Please use this identifier to cite or link to this item: https://doi.org/10.1534/genetics.104.038943
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dc.titleRecD plays an essential function during growth at low temperature in the antarctic bacterium Pseudomonas syringae Lz4W
dc.contributor.authorRegha, K
dc.contributor.authorSatapathy, AK
dc.contributor.authorRay, MK
dc.date.accessioned2022-01-10T03:26:26Z
dc.date.available2022-01-10T03:26:26Z
dc.date.issued2005-08-01
dc.identifier.citationRegha, K, Satapathy, AK, Ray, MK (2005-08-01). RecD plays an essential function during growth at low temperature in the antarctic bacterium Pseudomonas syringae Lz4W. Genetics 170 (4) : 1473-1484. ScholarBank@NUS Repository. https://doi.org/10.1534/genetics.104.038943
dc.identifier.issn00166731
dc.identifier.issn19432631
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/213516
dc.description.abstractThe Antarctic psychrotrophic bacterium Pseudomonas syringae Lz4W has been used as a model system to identify genes that are required for growth at low temperature. Transposon mutagenesis was carried out to isolate mutant(s) of the bacterium that are defective for growth at 4° but normal at 22°. In one such cold-sensitive mutant (CS1), the transposon-disrupted gene was identified to be a homolog of the recD gene of several bacteria. Trans-complementation and freshly targeted gene disruption studies reconfirmed that the inactivation of the recD gene leads to a cold-sensitive phenotype. We cloned, sequenced, and analyzed ∼11.2 kbp of DNA from recD and its flanking region from the bacterium. recD was the last gene of a putative recCBD operon. The RecD ORF was 694 amino acids long and 40% identical (52% similar) to the Escherichia coli protein, and it could complement the E. coli recD mutation. The recD gene of E. coli, however, could not complement the cold-sensitive phenotype of the CS1 mutant. Interestingly, the CS1 strain showed greater sensitivity toward the DNA-damaging agents, mitomycin C and UV. The inactivation of recD in P. syringae also led to cell death and accumulation of DNA fragments of ∼25-30 kbp in size at low temperature (4°). We propose that during growth at a very low temperature the Antarctic P. syringae is subjected to DNA damage, which requires direct participation of a unique RecD function. Additional results suggest that a truncated recD encoding the N-terminal segment of (1-576) amino acids is sufficient to support growth of P. syringae at low temperature. Copyright © 2005 by the Genetics Society of America.
dc.publisherOxford University Press (OUP)
dc.sourceElements
dc.subjectAlkylating Agents
dc.subjectAmino Acid Sequence
dc.subjectAntarctic Regions
dc.subjectApoptosis
dc.subjectCell Death
dc.subjectCloning, Molecular
dc.subjectCold Temperature
dc.subjectDNA
dc.subjectDNA Damage
dc.subjectDNA Transposable Elements
dc.subjectDrug Tolerance
dc.subjectEscherichia coli
dc.subjectEscherichia coli Proteins
dc.subjectGene Expression Regulation, Bacterial
dc.subjectGenes, Bacterial
dc.subjectGenetic Complementation Test
dc.subjectMitomycin
dc.subjectMolecular Sequence Data
dc.subjectMutagenesis, Site-Directed
dc.subjectMutation
dc.subjectOpen Reading Frames
dc.subjectOperon
dc.subjectPseudomonas syringae
dc.subjectRadiation Tolerance
dc.subjectSequence Analysis, DNA
dc.subjectSequence Homology, Amino Acid
dc.subjectUltraviolet Rays
dc.typeArticle
dc.date.updated2022-01-09T03:58:07Z
dc.contributor.departmentOPHTHALMOLOGY
dc.description.doi10.1534/genetics.104.038943
dc.description.sourcetitleGenetics
dc.description.volume170
dc.description.issue4
dc.description.page1473-1484
dc.published.statePublished
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