TiO2 AND Au NANOPARTICLES ASSISTED EPITHELIAL CELL EXTRUSION

Abstract

As a highly regulated layer to provide strong protection, previous studies have revealed that dead cells and alive cells can be squeezed out of the epithelial layer via cell extrusion. With the small sizes and diverse shapes, nanoparticles are nowadays being utilized in increasing fields. However, the effects of nanoparticle accumulation in the human body are primarily unknown. In this project, TiO2 and Au20 nanoparticles induced A431 cell extrusion had been discovered, named by Nanoparticles assisted cell extrusion (NanoACE). In addition, immunoprecipitation revealed the interactions between TiO2/Au20 nanoparticles and E-cadherin. In conclusion, this project has confirmed that NanoACE can be induced by TiO2 and Au20 nanoparticles in a concentration dependent manner. In the future, in vivo studies of NanoACE may assist in gaining more comprehensive results of apical extruded cell viability and metastasis, stating whether this phenomenon is beneficial or detrimental for tumor elimination ultimately.