Please use this identifier to cite or link to this item:
https://doi.org/10.1038/s41598-018-29073-7
DC Field | Value | |
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dc.title | Non-canonicaly recruited TCRαβCD8αα IELs recognize microbial antigens | |
dc.contributor.author | Wojciech, L. | |
dc.contributor.author | Szurek, E. | |
dc.contributor.author | Kuczma, M. | |
dc.contributor.author | Cebula, A. | |
dc.contributor.author | Elhefnawy, W.R. | |
dc.contributor.author | Pietrzak, M. | |
dc.contributor.author | Rempala, G. | |
dc.contributor.author | Ignatowicz, L. | |
dc.date.accessioned | 2021-12-29T05:49:17Z | |
dc.date.available | 2021-12-29T05:49:17Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Wojciech, L., Szurek, E., Kuczma, M., Cebula, A., Elhefnawy, W.R., Pietrzak, M., Rempala, G., Ignatowicz, L. (2018). Non-canonicaly recruited TCRαβCD8αα IELs recognize microbial antigens. Scientific Reports 8 (1) : 10848. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-018-29073-7 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/212527 | |
dc.description.abstract | In the gut, various subsets of intraepithelial T cells (IELs) respond to self or non-self-antigens derived from the body, diet, commensal and pathogenic microbiota. Dominant subset of IELs in the small intestine are TCRαβCD8αα+ cells, which are derived from immature thymocytes that express self-reactive TCRs. Although most of TCRαβCD8αα+ IELs are thymus-derived, their repertoire adapts to microbial flora. Here, using high throughput TCR sequencing we examined how clonal diversity of TCRαβCD8αα+ IELs changes upon exposure to commensal-derived antigens. We found that fraction of CD8αα+ IELs and CD4+ T cells express identical αβTCRs and this overlap raised parallel to a surge in the diversity of microbial flora. We also found that an opportunistic pathogen (Staphylococcus aureus) isolated from mouse small intestine specifically activated CD8αα+ IELs and CD4+ derived T cell hybridomas suggesting that some of TCRαβCD8αα+ clones with microbial specificities have extrathymic origin. We also report that CD8ααCD4+ IELs and Foxp3CD4+ T cells from the small intestine shared many αβTCRs, regardless whether the later subset was isolated from Foxp3CNS1 sufficient or Foxp3CNS1 deficient mice that lacks peripherally-derived Tregs. Overall, our results imply that repertoire of TCRαβCD8αα+ in small intestine expends in situ in response to changes in microbial flora. © 2018 The Author(s). | |
dc.publisher | Nature Publishing Group | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Scopus OA2018 | |
dc.type | Article | |
dc.contributor.department | MICROBIOLOGY AND IMMUNOLOGY | |
dc.description.doi | 10.1038/s41598-018-29073-7 | |
dc.description.sourcetitle | Scientific Reports | |
dc.description.volume | 8 | |
dc.description.issue | 1 | |
dc.description.page | 10848 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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