Please use this identifier to cite or link to this item: https://doi.org/10.3389/fmars.2019.00121
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dc.titleShort term exposure to heat and sediment triggers changes in coral gene expression and photo-physiological performance
dc.contributor.authorPoquita-Du, R.C.
dc.contributor.authorHuang, D.
dc.contributor.authorChou, L.M.
dc.contributor.authorMrinalini, Evolutionary Biology Laboratory, Department of Biological Sciences, National University of Singapore, Singapore, Singapore.
dc.contributor.authorTodd, P.A.
dc.date.accessioned2021-12-29T04:37:31Z
dc.date.available2021-12-29T04:37:31Z
dc.date.issued2019
dc.identifier.citationPoquita-Du, R.C., Huang, D., Chou, L.M., Mrinalini, Evolutionary Biology Laboratory, Department of Biological Sciences, National University of Singapore, Singapore, Singapore., Todd, P.A. (2019). Short term exposure to heat and sediment triggers changes in coral gene expression and photo-physiological performance. Frontiers in Marine Science 6 (MAR) : 121. ScholarBank@NUS Repository. https://doi.org/10.3389/fmars.2019.00121
dc.identifier.issn22967745
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/212335
dc.description.abstractCorals, together with their endosymbiotic Symbiodiniaceae, are known to exhibit a suite of mechanisms for survival under stressful conditions. However, exactly how the host animal responds to varying environmental conditions remains unclear. In this study, we tested two relevant environmental factors that can have deleterious effects on corals: heat and sediment. We examined among-genotype responses of Pocillopora acuta to these factors with RNA-Seq in concert with widely-used tools for assessing the physiological conditions of corals. Heat and sediment treatments were applied in a 2 × 2 crossed experimental design: (1) ~30°C without sediment (control, "C"), (2) ~30°C with sediment (sediment-only, "S"), (3) ~32°C without sediment (heat-only, "H"), (4) ~32°C with sediment (heat + sediment, "H+S") over four consecutive days (3-h daily exposure) in ex situ aquarium conditions. A clear differentiation in gene expression patterns was observed in corals exposed to heat alone and to heat with sediment, relative to the control treatment. Few transcripts (~3) were found to be differentially expressed for corals exposed to sediment only. The greater impact of heat was supported by photo-physiological measurements that showed significant effects on maximum quantum yield and average symbiont density among genotypes of P. acuta. The combined effect of heat and sediment caused a greater reduction in average symbiont density than the effect of sediment alone. Furthermore, "H+S" disrupted the ability of corals to maintain processes involving assembly and disassembly of cilium which suggests a synergistic effect between the two factors. We also found that host-specific genes which were expressed differentially may not be related to their interactions with algal symbionts. Rather, these genes are involved in a variety of biological functions including, but not limited to, cilium biogenesis and degradation, cilia motility, innate and adaptive immune responses, cell adhesion and bone mineralization, and processes involved in the cell cycle. These results reflect the complex response of the host alone. Overall, our findings indicate that acute heat stress in tandem with sediment can depress photo-physiological performance and trigger considerable changes in host gene expression. © 2019 Poquita-Du, Huang, Chou, Mrinalini and Todd.
dc.publisherFrontiers Media S.A.
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceScopus OA2019
dc.subjectEx situ experiment
dc.subjectGenotypes
dc.subjectPocillopora acuta
dc.subjectRNA-Seq
dc.subjectSymbiodiniaceae
dc.subjectTranscriptome-wide
dc.typeArticle
dc.contributor.departmentDEPT OF BIOLOGICAL SCIENCES
dc.contributor.departmentTROPICAL MARINE SCIENCE INSTITUTE
dc.description.doi10.3389/fmars.2019.00121
dc.description.sourcetitleFrontiers in Marine Science
dc.description.volume6
dc.description.issueMAR
dc.description.page121
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