Please use this identifier to cite or link to this item: https://doi.org/10.3390/ijms20246359
DC FieldValue
dc.titleProteomics analysis of early developmental stages of zebrafish embryos
dc.contributor.authorPurushothaman, K.
dc.contributor.authorDas, P.P.
dc.contributor.authorPresslauer, C.
dc.contributor.authorLim, T.K.
dc.contributor.authorJohansen, S.D.
dc.contributor.authorLin, Q.
dc.contributor.authorBabiak, I.
dc.date.accessioned2021-12-29T04:03:53Z
dc.date.available2021-12-29T04:03:53Z
dc.date.issued2019
dc.identifier.citationPurushothaman, K., Das, P.P., Presslauer, C., Lim, T.K., Johansen, S.D., Lin, Q., Babiak, I. (2019). Proteomics analysis of early developmental stages of zebrafish embryos. International Journal of Molecular Sciences 20 (24) : 6359. ScholarBank@NUS Repository. https://doi.org/10.3390/ijms20246359
dc.identifier.issn16616596
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/212218
dc.description.abstractZebrafish is a well-recognized organism for investigating vertebrate development and human diseases. However, the data on zebrafish proteome are scarce, particularly during embryogenesis. This is mostly due to the overwhelming abundance of egg yolk proteins, which tend to mask the detectable presence of less abundant proteins. We developed an efficient procedure to reduce the amount of yolk in zebrafish early embryos to improve the Liquid chromatography–tandem mass spectrometry (LC–MS)-based shotgun proteomics analysis. We demonstrated that the deyolking procedure resulted in a greater number of proteins being identified. This protocol resulted in approximately 2-fold increase in the number of proteins identified in deyolked samples at cleavage stages, and the number of identified proteins increased greatly by 3–4 times compared to non-deyolked samples in both oblong and bud stages. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed a high number of functional proteins differentially accumulated in the deyolked versus non-deyolked samples. The most prominent enrichments after the deyolking procedure included processes, functions, and components related to cellular organization, cell cycle, control of replication and translation, and mitochondrial functions. This deyolking procedure improves both qualitative and quantitative proteome analyses and provides an innovative tool in molecular embryogenesis of polylecithal animals, such as fish, amphibians, reptiles, or birds. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
dc.publisherMDPI AG
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceScopus OA2019
dc.subjectEgg yolk
dc.subjectEmbryonic development
dc.subjectLC
dc.subjectMS/MS shotgun proteomics
dc.subjectProteome
dc.subjectZebrafish
dc.typeArticle
dc.contributor.departmentDEPT OF BIOLOGICAL SCIENCES
dc.description.doi10.3390/ijms20246359
dc.description.sourcetitleInternational Journal of Molecular Sciences
dc.description.volume20
dc.description.issue24
dc.description.page6359
Appears in Collections:Staff Publications
Elements

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_3390_ijms20246359.pdf2.61 MBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

13
checked on Jan 25, 2023

Page view(s)

85
checked on Jan 26, 2023

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons