Please use this identifier to cite or link to this item: https://doi.org/10.3390/pharmaceutics13071052
DC FieldValue
dc.titleLyophilization Preserves the Intrinsic Cardioprotective Activity of Bioinspired Cell-Derived Nanovesicles
dc.contributor.authorNeupane, Yub Raj
dc.contributor.authorHuang, Chenyuan
dc.contributor.authorWang, Xiaoyuan
dc.contributor.authorChng, Wei Heng
dc.contributor.authorVenkatesan, Gopalakrishnan
dc.contributor.authorZharkova, Olga
dc.contributor.authorWacker, Matthias Gerhard
dc.contributor.authorCzarny, Bertrand
dc.contributor.authorStorm, Gerrit
dc.contributor.authorWang, Jiong-Wei
dc.contributor.authorPastorin, Giorgia
dc.date.accessioned2021-11-29T02:03:02Z
dc.date.available2021-11-29T02:03:02Z
dc.date.issued2021-07-01
dc.identifier.citationNeupane, Yub Raj, Huang, Chenyuan, Wang, Xiaoyuan, Chng, Wei Heng, Venkatesan, Gopalakrishnan, Zharkova, Olga, Wacker, Matthias Gerhard, Czarny, Bertrand, Storm, Gerrit, Wang, Jiong-Wei, Pastorin, Giorgia (2021-07-01). Lyophilization Preserves the Intrinsic Cardioprotective Activity of Bioinspired Cell-Derived Nanovesicles. PHARMACEUTICS 13 (7). ScholarBank@NUS Repository. https://doi.org/10.3390/pharmaceutics13071052
dc.identifier.issn19994923
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/208416
dc.description.abstractRecently, bioinspired cell-derived nanovesicles (CDNs) have gained much interest in the field of nanomedicine due to the preservation of biomolecular structure characteristics derived from their parent cells, which impart CDNs with unique properties in terms of binding and uptake by target cells and intrinsic biological activities. Although the production of CDNs can be easily and reproducibly achieved with any kind of cell culture, application of CDNs for therapeutic purposes has been greatly hampered by their physical and chemical instability during long-term storage in aqueous dispersion. In the present study, we conceived a lyophilization approach that would preserve critical characteristics regarding stability (vesicles’ size and protein content), structural integrity, and biological activity of CDNs for enabling long-term storage in freeze-dried form. Compared to the lyoprotectant sucrose, trehalose-lyoprotected CDNs showed significantly higher glass transition temperature and lower residual moisture content. As assessed by ATR-FTIR and far-UV circular dichroism, lyophilization in the presence of the lyoprotectant effectively maintained the secondary structure of cellular proteins. After reconstitution, lyoprotected CDNs were efficiently associated with HeLa cells, CT26 cells, and bone marrow-derived macrophages at a rate comparable to the freshly prepared CDNs. In vivo, both lyoprotected and freshly prepared CDNs, for the first time ever reported, targeted the injured heart, and exerted intrinsic cardioprotective effects within 24 h, attributable to the antioxidant capacity of CDNs in a myocardial ischemia/reperfusion injury animal model. Taken together, these results pave the way for further development of CDNs as cell-based therapeutics stabilized by lyophilization that enabled long-term storage while preserving their activity.
dc.language.isoen
dc.publisherMDPI
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectPharmacology & Pharmacy
dc.subjectcell-derived nanovesicles
dc.subjectexosome mimetics
dc.subjectbionanotechnology
dc.subjectlyophilization
dc.subjecttrehalose
dc.subjectcardioprotection
dc.subjectFORMULATION OPTIMIZATION
dc.subjectORAL DELIVERY
dc.subjectEX-VIVO
dc.subjectSTABILITY
dc.subjectLIPOSOMES
dc.subjectTREHALOSE
dc.subjectMEMBRANE
dc.subjectPROTEINS
dc.typeArticle
dc.date.updated2021-11-27T03:03:06Z
dc.contributor.departmentDEPT OF PHARMACY
dc.contributor.departmentDEPT OF SURGERY
dc.description.doi10.3390/pharmaceutics13071052
dc.description.sourcetitlePHARMACEUTICS
dc.description.volume13
dc.description.issue7
dc.published.statePublished
Appears in Collections:Staff Publications
Elements

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Lyophilization Preserves the Intrinsic Cardioprotective Activity of Bioinspired Cell-Derived Nanovesicles.pdf3.2 MBAdobe PDF

OPEN

NoneView/Download

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.