Please use this identifier to cite or link to this item: https://doi.org/10.3892/ol.2018.8462
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dc.titleBacillus Calmette-Guerin induces rapid gene expression changes in human bladder cancer cell lines that may modulate its survival
dc.contributor.authorRahmat, Juwita N
dc.contributor.authorEsuvaranathan, Kesavan
dc.contributor.authorMahendran, Ratha
dc.date.accessioned2021-11-26T14:31:14Z
dc.date.available2021-11-26T14:31:14Z
dc.date.issued2018-04-11
dc.identifier.citationRahmat, Juwita N, Esuvaranathan, Kesavan, Mahendran, Ratha (2018-04-11). Bacillus Calmette-Guerin induces rapid gene expression changes in human bladder cancer cell lines that may modulate its survival. ONCOLOGY LETTERS 15 (6) : 9231-9241. ScholarBank@NUS Repository. https://doi.org/10.3892/ol.2018.8462
dc.identifier.issn17921074
dc.identifier.issn17921082
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/208349
dc.description.abstractBacillus Calmette-Guérin (BCG) immunotherapy is the standard therapy for non-muscle invasive bladder cancer. The aim of the present study was to identify genes that are induced in response to BCG immunotherapy, as these may be potential biomarkers for the response to clinical therapy. To model clinical therapy, human bladder cancer cell lines were incubated with BCG (live or lyophilized BCG Connaught) for 2 h. RNA was extracted and evaluated by Representational Differential Analysis (RDA) and oligo arrays. Gene expression was confirmed by reverse transcription polymerase chain reaction on fresh cell lines with differential abilities to internalize BCG. The effect of 2 major BCG soluble proteins, antigen 85B (Ag85B) and Mycobacterium protein tyrosine phosphatase A (MptpA) and BCG Tice® on gene expression was also determined. GAPDH and β-actin, which are normally used as control genes, were upregulated by BCG. Therefore, the ribosomal RNA gene ribosomal protein S27a was used to normalize gene expression. The genes likely to be induced by BCG internalization and soluble factors were: GSTT2, MGST2, CCL20, TNFα, CCNE1 and IL10RB. Those induced by BCG membrane interactions and/or soluble factors were: MGST1, CXCL6, IL12A, CSF2, IL1β and TOLLIP. MptpA decreased GSTT2 expression, and Ag85B increased TNFα expression. The two BCG strains significantly increased GSTT2, TNFα and TOLLIP levels in MGH cells. However, in J82 cells there was a BCG strain-dependent difference in TNFα expression. An important outcome of the present study was the determination that neither GAPDH nor β-actin were suitable control genes for the analysis of BCG-induced gene expression. BCG Connaught and Tice® induced similar expression levels of genes in bladder cancer cell lines. BCG soluble proteins modulated gene expression and therefore may affect therapeutic outcomes. The genes identified may be novel biomarkers of the response to BCG therapy.
dc.language.isoen
dc.publisherSPANDIDOS PUBL LTD
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectOncology
dc.subjectBacillus Calmette-Guerin
dc.subjectgene expression
dc.subjectbladder cancer
dc.subjectcell lines
dc.subjectMYCOBACTERIUM-BOVIS
dc.subjectMURINE BLADDER
dc.subjectIMMUNOTHERAPY
dc.subjectMACROPHAGES
dc.subjectPATHWAY
dc.subjectTUMOR
dc.subjectINTERNALIZATION
dc.subjectINSTILLATION
dc.subjectACTIVATION
dc.subjectAUGMENTS
dc.typeArticle
dc.date.updated2021-11-26T05:16:46Z
dc.contributor.departmentDEPT OF BIOMEDICAL ENGINEERING
dc.contributor.departmentDEPT OF SURGERY
dc.description.doi10.3892/ol.2018.8462
dc.description.sourcetitleONCOLOGY LETTERS
dc.description.volume15
dc.description.issue6
dc.description.page9231-9241
dc.published.statePublished
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