Please use this identifier to cite or link to this item: https://doi.org/10.1242/dev.01854
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dc.titleTbx20 is essential for cardiac chamber differentiation and repression of Tbx2
dc.contributor.authorSingh, MK
dc.contributor.authorChristoffels, VM
dc.contributor.authorDias, JM
dc.contributor.authorTrowe, MO
dc.contributor.authorPetry, M
dc.contributor.authorSchuster-Gossler, K
dc.contributor.authorBurger, A
dc.contributor.authorEricson, J
dc.contributor.authorKispert, A
dc.date.accessioned2021-09-28T07:50:26Z
dc.date.available2021-09-28T07:50:26Z
dc.date.issued2005-06-01
dc.identifier.citationSingh, MK, Christoffels, VM, Dias, JM, Trowe, MO, Petry, M, Schuster-Gossler, K, Burger, A, Ericson, J, Kispert, A (2005-06-01). Tbx20 is essential for cardiac chamber differentiation and repression of Tbx2. DEVELOPMENT 132 (12) : 2697-2707. ScholarBank@NUS Repository. https://doi.org/10.1242/dev.01854
dc.identifier.issn09501991
dc.identifier.issn14779129
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/201431
dc.description.abstractTbx20, a member of the T-box family of transcriptional regulators, shows, evolutionary conserved expression in the developing heart. In the mouse, Tbx20 is expressed in the cardiac crescent, then in the endocardium and myocardium of the linear and looped heart tube before it is restricted to the atrioventricular canal and outflow tract in the multi-chambered heart. Here, we show that Tbx20 is required for progression from the linear heart tube to a multi-chambered heart. Mice carrying a targeted mutation of Tbx20 show early embryonic lethality due to hemodynamic failure. A linear heart tube with normal anteroposterior patterning is established in the mutant. The tube does not elongate, indicating a defect in recruitment of mesenchyme from the secondary heart field, even though markers of the secondary heart field are not affected. Furthermore, dorsoventral patterning of the tube, formation of working myocardium, looping, and further differentiation and morphogenesis fail. Instead, Tbx2, Bmp2 and vinexin α (Sh3d4), genes normally restricted to regions of primary myocardium and lining endocardium, are ectopically expressed in the linear heart tube of Tbx20 mutant embryos. Because Tbx2 is both necessary and sufficient to repress chamber differentiation (Christoffels et al., 2004a; Harrelson et al., 2004), Tbx20 may ensure progression to a multi-chambered heart by repressing Tbx2 in the myocardial precursor cells of the linear heart tube destined to form the chambers.
dc.language.isoen
dc.publisherCOMPANY BIOLOGISTS LTD
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectDevelopmental Biology
dc.subjectT-box
dc.subjectheart
dc.subjectmyocardium
dc.subjectanterior heart field
dc.subjectBmp
dc.subjectT-BOX GENE
dc.subjectBHLH TRANSCRIPTION FACTOR
dc.subjectEXPRESSION ANALYSIS
dc.subjectHEART
dc.subjectCELLS
dc.subjectMORPHOGENESIS
dc.subjectSPECIFICATION
dc.subjectANTERIOR
dc.subjectNKX2-5
dc.subjectEHAND
dc.typeArticle
dc.date.updated2021-09-22T02:40:43Z
dc.contributor.departmentCENTRE FOR ADVANCED 2D MATERIALS
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.description.doi10.1242/dev.01854
dc.description.sourcetitleDEVELOPMENT
dc.description.volume132
dc.description.issue12
dc.description.page2697-2707
dc.published.statePublished
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