Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.stemcr.2019.12.003
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dc.titleEnhancing the Efficacy of Stem Cell Therapy with Glycosaminoglycans
dc.contributor.authorLing, L.
dc.contributor.authorRen, X.
dc.contributor.authorCao, X.
dc.contributor.authorHassan, A.B.M.
dc.contributor.authorMah, S.
dc.contributor.authorSathiyanathan, P.
dc.contributor.authorSmith, R.A.A.
dc.contributor.authorTan, C.L.L.
dc.contributor.authorEio, M.
dc.contributor.authorSamsonraj, R.M.
dc.contributor.authorvan Wijnen, A.J.
dc.contributor.authorRaghunath, M.
dc.contributor.authorNurcombe, V.
dc.contributor.authorHui, J.H.
dc.contributor.authorCool, S.M.
dc.date.accessioned2021-08-10T03:05:29Z
dc.date.available2021-08-10T03:05:29Z
dc.date.issued2020
dc.identifier.citationLing, L., Ren, X., Cao, X., Hassan, A.B.M., Mah, S., Sathiyanathan, P., Smith, R.A.A., Tan, C.L.L., Eio, M., Samsonraj, R.M., van Wijnen, A.J., Raghunath, M., Nurcombe, V., Hui, J.H., Cool, S.M. (2020). Enhancing the Efficacy of Stem Cell Therapy with Glycosaminoglycans. Stem Cell Reports 14 (1) : 105-121. ScholarBank@NUS Repository. https://doi.org/10.1016/j.stemcr.2019.12.003
dc.identifier.issn2213-6711
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/196214
dc.description.abstractHuman mesenchymal stem cell (hMSC) therapy offers significant potential for osteochondral regeneration. Such applications require their ex vivo expansion in media frequently supplemented with fibroblast growth factor 2 (FGF2). Particular heparan sulfate (HS) fractions stabilize FGF2-FGF receptor complexes. We show that an FGF2-binding HS variant (HS8) accelerates the expansion of freshly isolated bone marrow hMSCs without compromising their naivety. Importantly, the repair of osteochondral defects in both rats and pigs is improved after treatment with HS8-supplemented hMSCs (MSCHS8), when assessed histologically, biomechanically, or by MRI. Thus, supplementing hMSC culture media with an HS variant that targets endogenously produced FGF2 allows the elimination of exogenous growth factors that may adversely affect their therapeutic potency. © 2019 The AuthorsThis study highlights a novel strategy to improve cartilage repair using hMSCs cultured with heparan sulfates that target and stabilize fibroblast growth factor 2. The work is focused on developing bio-additives that prevent the loss of hMSCs stemness during their ex vivo expansion that is important for maintaining their therapeutic utility. © 2019 The Authors
dc.publisherCell Press
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceScopus OA2020
dc.subjectcartilage repair
dc.subjectefficacy
dc.subjectex vivo expansion
dc.subjectFGF2
dc.subjectheparan sulfate
dc.subjectosteochondral regeneration
dc.subjectpotency
dc.subjectstemness
dc.typeArticle
dc.contributor.departmentORTHOPAEDIC SURGERY
dc.contributor.departmentBIOENGINEERING
dc.description.doi10.1016/j.stemcr.2019.12.003
dc.description.sourcetitleStem Cell Reports
dc.description.volume14
dc.description.issue1
dc.description.page105-121
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