Please use this identifier to cite or link to this item:
https://doi.org/10.3390/polym12030670
DC Field | Value | |
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dc.title | Inclusion of cross-linked elastin in gelatin/PEG hydrogels favourably influences fibroblast phenotype | |
dc.contributor.author | Cao, Y. | |
dc.contributor.author | Lee, B.H. | |
dc.contributor.author | Irvine, S.A. | |
dc.contributor.author | Wong, Y.S. | |
dc.contributor.author | Peled, H.B. | |
dc.contributor.author | Venkatraman, S. | |
dc.date.accessioned | 2021-08-10T03:04:29Z | |
dc.date.available | 2021-08-10T03:04:29Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Cao, Y., Lee, B.H., Irvine, S.A., Wong, Y.S., Peled, H.B., Venkatraman, S. (2020). Inclusion of cross-linked elastin in gelatin/PEG hydrogels favourably influences fibroblast phenotype. Polymers 12 (3) : 670. ScholarBank@NUS Repository. https://doi.org/10.3390/polym12030670 | |
dc.identifier.issn | 2073-4360 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/196195 | |
dc.description.abstract | The capacity of a biomaterial to innatelymodulate cell behaviorwhilemeeting themechanical property requirements of the implant is a much sought-after goal within bioengineering. Here we covalently incorporate soluble elastin into a gelatin-poly (ethylene glycol) (PEG) hydrogel for threedimensional (3D) cell encapsulation to achieve these properties. The inclusion of elastin into a previously optimized gelatin-PEG hydrogel was then evaluated for effects on entrapped fibroblasts, with the aim to assess the hydrogel as an extracellular matrix (ECM)-mimicking 3D microenvironment for cellular guidance. Soluble elastin was incorporated both physically and covalently into novel gelatin/elastin hybrid PEG hydrogels with the aim to harness the cellular interactivity and mechanical tunability of both elastin and gelatin. This design allowed us to assess the benefits of elastin-containing hydrogels in guiding fibroblast activity for evaluation as a potential dermal replacement. It was found that a gelatin-PEG hydrogel with covalently conjugated elastin, supported neonatal fibroblast viability, promoted their proliferation from 7.3% to 13.5% and guided their behavior. The expression of collagen alpha-1(COL1A1) and elastin in gelatin/elastin hybrid gels increased 16-fold and 6-fold compared to control sample at day 9, respectively. Moreover, cells can be loaded into the hydrogel precursor solution, deposited, and the matrix cross-linked without affecting the incorporated cells adversely, thus enabling a potential injectable system for dermal wound healing. © 2020 by the authors. | |
dc.publisher | MDPI AG | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Scopus OA2020 | |
dc.subject | Cell encapsulation | |
dc.subject | Dermal substitu | |
dc.subject | Elastin | |
dc.subject | Gelatin | |
dc.subject | Myofibroblast | |
dc.subject | Polyethylene glycol hydrogel | |
dc.type | Article | |
dc.contributor.department | INDUSTRY LIAISON OFFICE | |
dc.description.doi | 10.3390/polym12030670 | |
dc.description.sourcetitle | Polymers | |
dc.description.volume | 12 | |
dc.description.issue | 3 | |
dc.description.page | 670 | |
Appears in Collections: | Elements Staff Publications |
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