Please use this identifier to cite or link to this item: https://doi.org/10.3390/polym12030670
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dc.titleInclusion of cross-linked elastin in gelatin/PEG hydrogels favourably influences fibroblast phenotype
dc.contributor.authorCao, Y.
dc.contributor.authorLee, B.H.
dc.contributor.authorIrvine, S.A.
dc.contributor.authorWong, Y.S.
dc.contributor.authorPeled, H.B.
dc.contributor.authorVenkatraman, S.
dc.date.accessioned2021-08-10T03:04:29Z
dc.date.available2021-08-10T03:04:29Z
dc.date.issued2020
dc.identifier.citationCao, Y., Lee, B.H., Irvine, S.A., Wong, Y.S., Peled, H.B., Venkatraman, S. (2020). Inclusion of cross-linked elastin in gelatin/PEG hydrogels favourably influences fibroblast phenotype. Polymers 12 (3) : 670. ScholarBank@NUS Repository. https://doi.org/10.3390/polym12030670
dc.identifier.issn2073-4360
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/196195
dc.description.abstractThe capacity of a biomaterial to innatelymodulate cell behaviorwhilemeeting themechanical property requirements of the implant is a much sought-after goal within bioengineering. Here we covalently incorporate soluble elastin into a gelatin-poly (ethylene glycol) (PEG) hydrogel for threedimensional (3D) cell encapsulation to achieve these properties. The inclusion of elastin into a previously optimized gelatin-PEG hydrogel was then evaluated for effects on entrapped fibroblasts, with the aim to assess the hydrogel as an extracellular matrix (ECM)-mimicking 3D microenvironment for cellular guidance. Soluble elastin was incorporated both physically and covalently into novel gelatin/elastin hybrid PEG hydrogels with the aim to harness the cellular interactivity and mechanical tunability of both elastin and gelatin. This design allowed us to assess the benefits of elastin-containing hydrogels in guiding fibroblast activity for evaluation as a potential dermal replacement. It was found that a gelatin-PEG hydrogel with covalently conjugated elastin, supported neonatal fibroblast viability, promoted their proliferation from 7.3% to 13.5% and guided their behavior. The expression of collagen alpha-1(COL1A1) and elastin in gelatin/elastin hybrid gels increased 16-fold and 6-fold compared to control sample at day 9, respectively. Moreover, cells can be loaded into the hydrogel precursor solution, deposited, and the matrix cross-linked without affecting the incorporated cells adversely, thus enabling a potential injectable system for dermal wound healing. © 2020 by the authors.
dc.publisherMDPI AG
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceScopus OA2020
dc.subjectCell encapsulation
dc.subjectDermal substitu
dc.subjectElastin
dc.subjectGelatin
dc.subjectMyofibroblast
dc.subjectPolyethylene glycol hydrogel
dc.typeArticle
dc.contributor.departmentINDUSTRY LIAISON OFFICE
dc.description.doi10.3390/polym12030670
dc.description.sourcetitlePolymers
dc.description.volume12
dc.description.issue3
dc.description.page670
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