Please use this identifier to cite or link to this item: https://doi.org/10.3389/fimmu.2020.01190
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dc.titleEomes Expression Defines Group 1 Innate Lymphoid Cells During Metastasis in Human and Mouse
dc.contributor.authorVerma, Riva
dc.contributor.authorEr, Jun Zhi
dc.contributor.authorPu, Ren Wei
dc.contributor.authorMohamed, Jameelah Sheik
dc.contributor.authorSoo, Ross A
dc.contributor.authorMuthiah, Harish Mithiran
dc.contributor.authorTam, John Kit Chung
dc.contributor.authorDing, Jeak Ling
dc.date.accessioned2021-07-06T11:59:32Z
dc.date.available2021-07-06T11:59:32Z
dc.date.issued2020-06-17
dc.identifier.citationVerma, Riva, Er, Jun Zhi, Pu, Ren Wei, Mohamed, Jameelah Sheik, Soo, Ross A, Muthiah, Harish Mithiran, Tam, John Kit Chung, Ding, Jeak Ling (2020-06-17). Eomes Expression Defines Group 1 Innate Lymphoid Cells During Metastasis in Human and Mouse. FRONTIERS IN IMMUNOLOGY 11. ScholarBank@NUS Repository. https://doi.org/10.3389/fimmu.2020.01190
dc.identifier.issn16643224
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/193725
dc.description.abstractRecent studies have attempted to uncover the role of Group 1 Innate lymphoid cells (ILCs) in multiple physiological contexts, including cancer. However, the definition and precise contribution of Group 1 ILCs (constituting ILC1 and NK subsets) to metastasis is unclear due to the lack of well-defined cell markers. Here, we first identified ILC1 and NK cells in NSCLC patient blood and differentiated them based on the expression of transcription factors, T-bet and Eomes. Interestingly, Eomes downregulation in the peripheral blood NK cells of NSCLC patients positively correlated with disease progression. Additionally, we noted higher Eomes expression in NK cells (T-bet+Eomeshi) compared to ILC1s (T-bet+Eomeslo). We asked whether the decrease in Eomes was associated with the conversion of NK cells into ILC1 using Eomes as a reliable marker to differentiate ILC1s from NK cells. Utilizing a murine model of experimental metastasis, we observed an association between increase in metastasis and Eomes downregulation in NKp46+NK1.1+ Group 1 ILCs, which was consistent to that of human NSCLC samples. Further confirmation of this trend was achieved by flow cytometry, which identified tissue-specific Eomeslo ILC1-like and Eomeshi NK-like subsets in the murine metastatic lung based on cell surface markers and adoptive transfer experiments. Next, functional characterization of these cell subsets showed reduced cytotoxicity and IFNγ production in Eomeslo ILC1s compared to Eomeshi cells, suggesting that lower Eomes levels are associated with poor cancer immunosurveillance by Group 1 ILCs. These findings provide novel insights into the regulation of Group 1 ILC subsets during metastasis, through the use of Eomes as a reliable marker to differentiate between NK and ILC1s.
dc.language.isoen
dc.publisherFRONTIERS MEDIA SA
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectImmunology
dc.subjectEomesodermin
dc.subjectGroup 1 ILCs
dc.subjectInnate Lymphoid Cells
dc.subjectmetastasis
dc.subjectnon-small cell lung cancer
dc.subjectT-BET
dc.subjectNK CELLS
dc.subjectHOMEOSTASIS
dc.subjectDIFFERENTIATION
dc.subjectPLASTICITY
dc.subjectPHENOTYPE
dc.subjectINSTRUCT
dc.subjectLIVER
dc.typeArticle
dc.date.updated2021-07-06T07:41:33Z
dc.contributor.departmentCANCER SCIENCE INSTITUTE OF SINGAPORE
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.contributor.departmentSURGERY
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.description.doi10.3389/fimmu.2020.01190
dc.description.sourcetitleFRONTIERS IN IMMUNOLOGY
dc.description.volume11
dc.published.statePublished
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