Please use this identifier to cite or link to this item: https://doi.org/10.22203/eCM.v041a16
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dc.titlePULSED ELECTROMAGNETIC FIELDS SYNERGIZE WITH GRAPHENE TO ENHANCE DENTAL PULP STEM CELL-DERIVED NEUROGENESIS BY SELECTIVELY TARGETING TRPC1 CHANNELS
dc.contributor.authorMadanagopal, TT
dc.contributor.authorTai, YK
dc.contributor.authorLim, SH
dc.contributor.authorFong, CHH
dc.contributor.authorCao, T
dc.contributor.authorRosa, V
dc.contributor.authorFranco-Obregon, A
dc.date.accessioned2021-07-02T06:27:07Z
dc.date.available2021-07-02T06:27:07Z
dc.date.issued2021-01-01
dc.identifier.citationMadanagopal, TT, Tai, YK, Lim, SH, Fong, CHH, Cao, T, Rosa, V, Franco-Obregon, A (2021-01-01). PULSED ELECTROMAGNETIC FIELDS SYNERGIZE WITH GRAPHENE TO ENHANCE DENTAL PULP STEM CELL-DERIVED NEUROGENESIS BY SELECTIVELY TARGETING TRPC1 CHANNELS. EUROPEAN CELLS & MATERIALS 41 : 216-232. ScholarBank@NUS Repository. https://doi.org/10.22203/eCM.v041a16
dc.identifier.issn14732262
dc.identifier.issn14732262
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/193221
dc.description.abstractConventional root canal treatment replaces the infected pulp with defined materials. Alternative cell-based tissue engineering strategies aim to regenerate a fully functional pulp within the root canal. Despite recent advances in this area, however, the regeneration of an innervated pulp remains a major challenge in the field. Both graphene (2DG) and pulsed electromagnetic fields (PEMFs) independently have been shown to promote diverse cellular developmental programs. The present study showed that 2DG promoted the neurogenic induction of human dental pulp stem cells (hDPSCs) by upregulating and accelerating the expression of mature neuronal markers. Notably, 2DG induced the highest expression of transient receptor potential canonical cation channel type 1 (TRPC1) during early neurogenesis. As brief PEMF exposure promotes in vitro differentiation by activating a TRPC1-mitochondrial axis, an opportunity to combine 2DG with developmentally targeted PEMF exposure for synergistic effects was realizable. Neurogenic gene expression, neurotransmitter release, and reactive oxygen species (ROS) production were greatly enhanced by a brief (10 min) and low amplitude (2 mT) PEMF exposure timed to coincide with the highest TRPC1 expression from hDPSCs on 2DG. In contrast, hDPSCs on glass were less responsive to PEMF exposure. The capacity of PEMFs to promote neurogenesis was precluded by the administration of penicillin/streptomycin, mirroring previous studies demonstrating that aminoglycoside antibiotics block TRPC1-mediated calcium entry and verifying the contribution of TRPC1 in this form of magnetoreception. Hence, graphene created a more conducive environment for subsequent PEMF-stimulated neurogenic induction of hDPSCs through their mutual capacity to activate TRPC1with subsequent ROS production.
dc.language.isoen
dc.publisherAO RESEARCH INSTITUTE DAVOS-ARI
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectTechnology
dc.subjectCell & Tissue Engineering
dc.subjectEngineering, Biomedical
dc.subjectMaterials Science, Biomaterials
dc.subjectOrthopedics
dc.subjectCell Biology
dc.subjectEngineering
dc.subjectMaterials Science
dc.subjectPulsed electromagnetic fields
dc.subjectmitohormesis
dc.subjecttissue engineering
dc.subjectnanomaterial
dc.subjectTRPM8 CHANNEL
dc.subjectDIFFERENTIATION
dc.subjectBIOMATERIALS
dc.subjectMUTATIONS
dc.subjectAPOPTOSIS
dc.subjectNEURONS
dc.subjectGROWTH
dc.typeArticle
dc.date.updated2021-07-02T06:15:42Z
dc.contributor.departmentBIOCHEMISTRY
dc.contributor.departmentDENTISTRY
dc.contributor.departmentMECHANICAL ENGINEERING
dc.contributor.departmentSURGERY
dc.contributor.departmentSINGAPORE-MIT ALLIANCE
dc.description.doi10.22203/eCM.v041a16
dc.description.sourcetitleEUROPEAN CELLS & MATERIALS
dc.description.volume41
dc.description.page216-232
dc.published.statePublished
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