THE REGULATORY ROLE OF ENERGY METABOLISM DURING THE GERMINATION AND STORAGE OF ORGANIC SPROUTS AND THEIR SAFETY CONTROL BY LACTIC ACID BASED STRATEGIES

Abstract

In this study, the regulatory role of energy statues during the germination of organic mung bean and broccoli was investigated. The exogenous ATP effectively enhanced the intracellular ATP and energy charge. As a result, the germination was promoted and the biomass was accumulated. Further metabolomics study revealed the dramatic metabolic changes during the germination of mung bean, and the key factor (VrFBPase) involved in the reprogramming was screened and characterised. To improve the safety of organic sprouts under National Organic Program regulation, the lactic acid based sanitising strategies were further studied. In addition, the combination of lactic acid with some mild treatments such as low concentration sodium hypochlorite or mild heat further improved the disinfectant efficacies. Metabolic and transcriptional analysis showed that the glutamic acid dependent decarboxylase/antiporter system contributed to the lactic acid stress adaption ability of pathogenic strains.