Please use this identifier to cite or link to this item:
https://doi.org/10.1038/srep26339
DC Field | Value | |
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dc.title | Decellularization of human stromal refractive lenticules for corneal tissue engineering | |
dc.contributor.author | Yam, G.H.-F | |
dc.contributor.author | Yusoff, N.Z.B.M | |
dc.contributor.author | Goh, T.-W | |
dc.contributor.author | Setiawan, M | |
dc.contributor.author | Lee, X.-W | |
dc.contributor.author | Liu, Y.-C | |
dc.contributor.author | Mehta, J.S | |
dc.date.accessioned | 2020-10-31T11:34:09Z | |
dc.date.available | 2020-10-31T11:34:09Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Yam, G.H.-F, Yusoff, N.Z.B.M, Goh, T.-W, Setiawan, M, Lee, X.-W, Liu, Y.-C, Mehta, J.S (2016). Decellularization of human stromal refractive lenticules for corneal tissue engineering. Scientific Reports 6 : 26339. ScholarBank@NUS Repository. https://doi.org/10.1038/srep26339 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/182470 | |
dc.description.abstract | Small incision lenticule extraction (SMILE) becomes a procedure to correct myopia. The extracted lenticule can be used for other clinical scenarios. To prepare for allogeneic implantation, lenticule decellularization with preserved optical property, stromal architecture and chemistry would be necessary. We evaluated different methods to decellularize thin human corneal stromal lenticules created by femtosecond laser. Treatment with 0.1% sodium dodecylsulfate (SDS) followed by extensive washes was the most efficient protocol to remove cellular and nuclear materials. Empty cell space was found inside the stroma, which displayed aligned collagen fibril architecture similar to native stroma. The SDS-based method was superior to other treatments with hyperosmotic 1.5 M sodium chloride, 0.1% Triton X-100 and nucleases (from 2 to 10 U/ml DNase and RNase) in preserving extracellular matrix content (collagens, glycoproteins and glycosaminoglycans). The stromal transparency and light transmittance was indifferent to untreated lenticules. In vitro recellularization showed that the SDS-treated lenticules supported corneal stromal fibroblast growth. In vivo re-implantation into a rabbit stromal pocket further revealed the safety and biocompatibility of SDS-decellularized lenticules without short- and long-term rejection risk. Our results concluded that femtosecond laser-derived human stromal lenticules decellularized by 0.1% SDS could generate a transplantable bioscaffold with native-like stromal architecture and chemistry. | |
dc.publisher | Nature Publishing Group | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20201031 | |
dc.subject | dodecyl sulfate sodium | |
dc.subject | allotransplantation | |
dc.subject | animal | |
dc.subject | chemistry | |
dc.subject | cornea | |
dc.subject | cornea stroma | |
dc.subject | cornea transplantation | |
dc.subject | cytology | |
dc.subject | disease model | |
dc.subject | drug effects | |
dc.subject | human | |
dc.subject | Leporidae | |
dc.subject | photorefractive keratectomy | |
dc.subject | procedures | |
dc.subject | surgery | |
dc.subject | tissue engineering | |
dc.subject | transplantation | |
dc.subject | Animals | |
dc.subject | Cornea | |
dc.subject | Corneal Stroma | |
dc.subject | Corneal Surgery, Laser | |
dc.subject | Corneal Transplantation | |
dc.subject | Disease Models, Animal | |
dc.subject | Humans | |
dc.subject | Rabbits | |
dc.subject | Sodium Dodecyl Sulfate | |
dc.subject | Tissue and Organ Harvesting | |
dc.subject | Tissue Engineering | |
dc.subject | Transplantation, Homologous | |
dc.type | Article | |
dc.contributor.department | DUKE-NUS MEDICAL SCHOOL | |
dc.description.doi | 10.1038/srep26339 | |
dc.description.sourcetitle | Scientific Reports | |
dc.description.volume | 6 | |
dc.description.page | 26339 | |
dc.published.state | published | |
Appears in Collections: | Elements Staff Publications |
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