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Please use this identifier to cite or link to this item: https://doi.org/10.1186/s12944-015-0163-6
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dc.titleDDX5/p68 RNA helicase expression is essential for initiating adipogenesis
dc.contributor.authorRamanathan, N
dc.contributor.authorLim, N
dc.contributor.authorStewart, C.L
dc.date.accessioned2020-10-27T10:51:14Z
dc.date.available2020-10-27T10:51:14Z
dc.date.issued2015
dc.identifier.citationRamanathan, N, Lim, N, Stewart, C.L (2015). DDX5/p68 RNA helicase expression is essential for initiating adipogenesis. Lipids in Health and Disease 14 (1) : 160. ScholarBank@NUS Repository. https://doi.org/10.1186/s12944-015-0163-6
dc.identifier.issn1476511X
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/181415
dc.description.abstractBackground: DDX5/p68 RNA helicase is a member of the DEAD (Asp-Glu-Ala-Asp) box proteins. Apart from RNA unwinding, DDX5 is an important transcriptional factor and co-activator in cell proliferation and differentiation. Findings: Here, we have characterised the role of DDX5 in adipogenesis in 3T3-L1 cells using siRNA mediated suppression. Transient inhibition of Ddx5 mRNA expression at the start of adipogenesis impairs the differentiation programme even when DDX5 expression is restored later in adipogenesis. However transient suppression of Ddx5 at the later stages of adipogenesis do not impair adipogenesis or triglyceride accumulation suggesting Ddx5 expression is dispensable in a mature adipocyte. Conclusion: These results implicate DDX5 as a crucial factor involved in the complex transcriptional cascade of events that regulate adipogenesis and essential to the initiation of adipogenesis. © 2015 Ramanathan et al.
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20201031
dc.subjectDEAD box protein 5
dc.subjectnuclear factor
dc.subjectunclassified drug
dc.subjectDdx5 protein, mouse
dc.subjectDEAD box protein
dc.subjectadipocyte
dc.subjectadipogenesis
dc.subjectanimal cell
dc.subjectArticle
dc.subjectcell differentiation
dc.subjectcell viability
dc.subjectcontrolled study
dc.subjectDdx5 gene
dc.subjectgene
dc.subjectgene expression
dc.subjectgene function
dc.subjectgene repression
dc.subjectnonhuman
dc.subjectprotein expression
dc.subjectRNA isolation
dc.subject3T3-L1 cell line
dc.subjectanimal
dc.subjectC3H mouse
dc.subjectenzymology
dc.subjectgene silencing
dc.subjectgenetics
dc.subjectmesenchymal stroma cell
dc.subjectmetabolism
dc.subjectmouse
dc.subjectphysiology
dc.subject3T3-L1 Cells
dc.subjectAdipocytes
dc.subjectAdipogenesis
dc.subjectAnimals
dc.subjectDEAD-box RNA Helicases
dc.subjectGene Expression
dc.subjectGene Knockdown Techniques
dc.subjectMesenchymal Stromal Cells
dc.subjectMice
dc.subjectMice, Inbred C3H
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1186/s12944-015-0163-6
dc.description.sourcetitleLipids in Health and Disease
dc.description.volume14
dc.description.issue1
dc.description.page160
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