Please use this identifier to cite or link to this item: https://doi.org/10.1158/1078-0432.CCR-04-1944
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dc.titleIn vitro canine distemper virus infection of canine lymphoid cells: A prelude to oncolytic therapy for lymphoma
dc.contributor.authorSuter, S.E
dc.contributor.authorChein, M.B
dc.contributor.authorVERONIKA ALICE IRMELA VON MESS
dc.contributor.authorYip, B
dc.contributor.authorCattaneo, R
dc.contributor.authorVernau, W
dc.contributor.authorMadewell, B.R
dc.contributor.authorLondon, C.A
dc.date.accessioned2020-10-27T09:49:24Z
dc.date.available2020-10-27T09:49:24Z
dc.date.issued2005
dc.identifier.citationSuter, S.E, Chein, M.B, VERONIKA ALICE IRMELA VON MESS, Yip, B, Cattaneo, R, Vernau, W, Madewell, B.R, London, C.A (2005). In vitro canine distemper virus infection of canine lymphoid cells: A prelude to oncolytic therapy for lymphoma. Clinical Cancer Research 11 (4) : 1579-1587. ScholarBank@NUS Repository. https://doi.org/10.1158/1078-0432.CCR-04-1944
dc.identifier.issn10780432
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/181096
dc.description.abstractPurpose: Measles virus (MV) causes the regression of human lymphoma xenografts. The purpose of this study was to determine if canine lymphoid cells could be infected in vitro with MV or canine distemper virus (CDV, the canine Morbillivirus equivalent of MV) and determine if in vitro viral infection leads to apoptotic cell death. Experimental Design: Reverse transcriptase-PCR was used to examine the expression of both signal lymphocyte activation molecule (CD150) and membrane cofactor molecule (CD46) mRNA. An attenuated CDV expressing enhanced green fluorescent protein was used to infect canine cells in vitro. Both flow cytometry and reverse transcriptase-PCR was used to document CDV infection. Cell death was examined using a propidium iodide staining assay and Annexin V binding. Results: Canine lymphoid cell lines and neoplastic B and T lymphocytes collected from dogs with spontaneous lymphoma expressed the Morbillivirus receptor CD150 mRNA. In contrast, only neoplastic lymphocytes expressed detectable levels of CD46 mRNA. Although MV did not infect canine cells, CDV efficiently infected between 40% and 70% of all three canine lymphoid lines tested. More importantly, CDV infected 50% to 90% of neoplastic lymphocytes isolated from dogs with both B and T cell lymphoma. Apoptosis of CDV-infected cell lines was documented. Conclusions: Attenuated CDV may be a useful treatment for canine lymphoma. As such, dogs with lymphoma may represent a biologically relevant large animal model to investigate the feasibility, safety, and efficacy of Morbillivirus therapy in a clinical setting with findings that may have direct applicability in the treatment of human non-Hodgkin's lymphoma. © 2005 American Association for Cancer Research.
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20201031
dc.subjectCD150 antigen
dc.subjectenhanced green fluorescent protein
dc.subjectlipocortin 5
dc.subjectmembrane cofactor protein
dc.subjectmessenger RNA
dc.subjectpropidium iodide
dc.subjectanimal cell
dc.subjectantineoplastic activity
dc.subjectapoptosis
dc.subjectarticle
dc.subjectB lymphocyte
dc.subjectCanine distemper morbillivirus
dc.subjectcell death
dc.subjectdog
dc.subjectdog disease
dc.subjectflow cytometry
dc.subjecthuman
dc.subjecthuman cell
dc.subjectin vitro study
dc.subjectlymphoid cell
dc.subjectlymphoma
dc.subjectnonhodgkin lymphoma
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectpriority journal
dc.subjectprotein binding
dc.subjectprotein expression
dc.subjectreverse transcription polymerase chain reaction
dc.subjectT lymphocyte
dc.subjecttreatment indication
dc.subjectvirus attenuation
dc.subjectAnimals
dc.subjectAntigens, CD
dc.subjectAntigens, CD46
dc.subjectApoptosis
dc.subjectCell Line, Tumor
dc.subjectCercopithecus aethiops
dc.subjectDistemper Virus, Canine
dc.subjectDogs
dc.subjectFlow Cytometry
dc.subjectGene Expression
dc.subjectGlycoproteins
dc.subjectGreen Fluorescent Proteins
dc.subjectHumans
dc.subjectImmunoglobulins
dc.subjectJurkat Cells
dc.subjectLymphocytes
dc.subjectLymphoma
dc.subjectMembrane Glycoproteins
dc.subjectPlasmids
dc.subjectReceptors, Cell Surface
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectRNA, Messenger
dc.subjectTransfection
dc.subjectVero Cells
dc.typeArticle
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.description.doi10.1158/1078-0432.CCR-04-1944
dc.description.sourcetitleClinical Cancer Research
dc.description.volume11
dc.description.issue4
dc.description.page1579-1587
dc.published.stateUnpublished
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