Please use this identifier to cite or link to this item:
https://doi.org/10.1242/jcs.03387
DC Field | Value | |
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dc.title | VAMP4 cycles from the cell surface to the trans-Golgi network via sorting and recycling endosomes | |
dc.contributor.author | Hoai, T | |
dc.contributor.author | Tran, T | |
dc.contributor.author | Zeng, Q | |
dc.contributor.author | Hong, W | |
dc.date.accessioned | 2020-10-27T07:00:27Z | |
dc.date.available | 2020-10-27T07:00:27Z | |
dc.date.issued | 2007 | |
dc.identifier.citation | Hoai, T, Tran, T, Zeng, Q, Hong, W (2007). VAMP4 cycles from the cell surface to the trans-Golgi network via sorting and recycling endosomes. Journal of Cell Science 120 (6) : 1028-1041. ScholarBank@NUS Repository. https://doi.org/10.1242/jcs.03387 | |
dc.identifier.issn | 0021-9533 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/181041 | |
dc.description.abstract | VAMP4 is enriched in the trans-Golgi network (TGN) and functions in traffic from the early and recycling endosomes to the TGN, but its trafficking itinerary is unknown. Cells stably expressing TGN-enriched VAMP4 C-terminally-tagged with EGFP (VAMP4-EGFP) are able to internalize and transport EGFP antibody efficiently to the TGN, suggesting that VAMP4-EGFP cycles between the cell surface and the TGN. The N-terminal extension of VAMP4 endows a chimeric VAMP5 with the ability to cycle from the surface to the TGN. Detailed time-course analysis of EGFP antibody transport to the TGN as well as pharmacological and thermal perturbation experiments suggest that VAMP4-EGFP is endocytosed by clathrin-dependent pathways and is delivered to the sorting and then recycling endosomes. This is followed by a direct transport to the TGN, without going through the late endosome. The di-Leu motif of the TGN-targeting signal is important for internalization, whereas the acidic cluster is crucial for efficient delivery of internalized antibody from the endosome to the TGN. These results suggest that the TGN-targeting signal of VAMP4 mediates the efficient recycling of VAMP4 from the cell surface to the TGN via the sorting and recycling endosomes, thus conferring steady-state enrichment of VAMP4 at the TGN. | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20201031 | |
dc.subject | chimeric protein | |
dc.subject | clathrin | |
dc.subject | enhanced green fluorescent protein | |
dc.subject | SNARE protein | |
dc.subject | unclassified drug | |
dc.subject | VAMP4 protein | |
dc.subject | VAMP5 protein | |
dc.subject | amino terminal sequence | |
dc.subject | animal cell | |
dc.subject | article | |
dc.subject | carboxy terminal sequence | |
dc.subject | cell surface | |
dc.subject | cell vacuole | |
dc.subject | controlled study | |
dc.subject | endocytosis | |
dc.subject | endosome | |
dc.subject | internalization | |
dc.subject | intracellular transport | |
dc.subject | nonhuman | |
dc.subject | priority journal | |
dc.subject | protein expression | |
dc.subject | protein function | |
dc.subject | protein localization | |
dc.subject | protein motif | |
dc.subject | protein transport | |
dc.subject | rat | |
dc.subject | signal transduction | |
dc.subject | steady state | |
dc.subject | time | |
dc.subject | trans Golgi network | |
dc.subject | Animals | |
dc.subject | Cell Line | |
dc.subject | Cell Membrane | |
dc.subject | Cercopithecus aethiops | |
dc.subject | Endocytosis | |
dc.subject | Endosomes | |
dc.subject | Golgi Apparatus | |
dc.subject | Green Fluorescent Proteins | |
dc.subject | Macrolides | |
dc.subject | Mutation | |
dc.subject | Protein Sorting Signals | |
dc.subject | Protein Transport | |
dc.subject | R-SNARE Proteins | |
dc.subject | Rats | |
dc.subject | Recombinant Fusion Proteins | |
dc.type | Article | |
dc.contributor.department | BIOCHEMISTRY | |
dc.contributor.department | INSTITUTE OF MOLECULAR & CELL BIOLOGY | |
dc.description.doi | 10.1242/jcs.03387 | |
dc.description.sourcetitle | Journal of Cell Science | |
dc.description.volume | 120 | |
dc.description.issue | 6 | |
dc.description.page | 1028-1041 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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