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https://doi.org/10.3389/fmicb.2015.01445
Title: | Comparative genomics of two ST 195 carbapenem-resistant Acinetobacter baumannii with different susceptibility to polymyxin revealed underlying resistance mechanism | Authors: | Lean, S.-S Yeo, C.C Suhaili, Z Thong, K.-L |
Keywords: | aminoglycoside aztreonam bacterium lipopolysaccharide cefepime ceftazidime cephalosporinase DNA topoisomerase (ATP hydrolysing) DNA topoisomerase IV imipenem macrolide polymyxin streptomycin tetracycline Acinetobacter baumannii antibiotic sensitivity Article bacterial chromosome bacterial genome biosynthesis carbapenem resistant Acinetobacter baumannii controlled study DNA extraction gene mutation gene overexpression gene sequence genomics minimum inhibitory concentration molecular cloning multilocus sequence typing nonhuman nucleotide sequence phylogeny plasmid polyacrylamide gel electrophoresis real time polymerase chain reaction signal transduction |
Issue Date: | 2016 | Citation: | Lean, S.-S, Yeo, C.C, Suhaili, Z, Thong, K.-L (2016). Comparative genomics of two ST 195 carbapenem-resistant Acinetobacter baumannii with different susceptibility to polymyxin revealed underlying resistance mechanism. Frontiers in Microbiology 6 (JAN) : 1445. ScholarBank@NUS Repository. https://doi.org/10.3389/fmicb.2015.01445 | Rights: | Attribution 4.0 International | Abstract: | Acinetobacter baumannii is a Gram-negative nosocomial pathogen of importance due to its uncanny ability to acquire resistance to most antimicrobials. These include carbapenems, which are the drugs of choice for treating A. baumannii infections, and polymyxins, the drugs of last resort. Whole genome sequencing was performed on two clinical carbapenem-resistant A. baumannii AC29 and AC30 strains which had an indistinguishable ApaI pulsotype but different susceptibilities to polymyxin. Both genomes consisted of an approximately 3.8 Mbp circular chromosome each and several plasmids. AC29 (susceptible to polymyxin) and AC30 (resistant to polymyxin) belonged to the ST195 lineage and are phylogenetically clustered under the International Clone II (IC-II) group. An AbaR4-type resistance island (RI) interrupted the comM gene in the chromosomes of both strains and contained the blaOXA-23 carbapenemase gene and determinants for tetracycline and streptomycin resistance. AC29 harbored another copy of blaOXA-23 in a large (~74 kb) conjugative plasmid, pAC29b, but this gene was absent in a similar plasmid (pAC30c) found in AC30. A 7 kb Tn1548 | Source Title: | Frontiers in Microbiology | URI: | https://scholarbank.nus.edu.sg/handle/10635/180866 | ISSN: | 1664302X | DOI: | 10.3389/fmicb.2015.01445 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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